Acute Intestinal Obstruction In Children With Intestinal Mucosal Immune Barrier Damage And Bacterial Translocation Studies

objectives To investigate bacterial translocationand its mechanis in children with the acute intestinal obstructionMethods Polymerase chain reaction(PCR)was performed after DNA extraction,with targetβ-1actosidase gene(BG)of E.coil and 16SrRNA gene of most pathogenic bacteria.Bacteria in mesenteric lymph nodes were cultured;T lymphocyte subsets and plasma cell count in intestinal mucosa lamina propria were tested by immunohistochemical assay;Enzyme-linked immunosorbent assay (ELISA) was used to detect the level of TGF-β1 in serum;Enzyme-linked immunosorbent assay(ELISA) was used to detect the levelof interleukin-10 (IL-10) in serum.Result In the bowel resection anastomosis group and without bowel resection group,16SrRNA were positive in86.4%and 64.3%of the patients respectively,BG were positive in 72.7%and 42.8%respectively,positive cuhural rate of E coli in mesenteric lymph nodes was 77.3 % and57.1 % .In the conservative treatment group,16SrRNA were positive in57.1 % of the patients,BG were positive in 28.5%.Bacteria DNA were not detected in the serum control group;In the bowel resection anastomosis group,T cell subsets and plasma cells in intestinal mucosa lamina propria were lower than the normal control group;In the bowel resection anastomosis group and without bowel resection group,level of TGF-β1 in serum before surgery were both higher than the conservative treatment group and control group.In all the groups,level of TGF-β1 in 7 days after surgery were no difference compared with control group;In the three groups of Intestinal obstruction,level of IL-10 in serum before surgery were all higher than the control group(P<0.01);there were difference within group;level of IL-10 in serum before surgery were different from IL-10 in 7 days after surgery in the three groups of Intestinal obstructionConclusion T cell subsets and plasma cells in the lamina propria were reduced in children with acute intestinal obstruction, which caused damage to intestinal mucosal immune barrier; meanwhile the TGF-β1 and IL-10 which participation in immune suppression increased destruction of mucosal immune barrier,and Organism susceptibility, and promoted the bacterial translocation.