CDX-2 And LI-cadherin In Human Gastric Cancer Tissues And Significance

Objective: CDX-2 and LI-cadherin in gastric cancer and precancerous lesions of the situation and in case of gastric cancer in epithelial expression and distribution are still unclear, through research the CDX-2 and LI-cadherin expression levels and distribution in gastric cancer, adjacent, normal gastric mucosa, Gastrointestinal stromal tumors, chronic gastritis and gastric intestinal metaplasia, and both the expression of different gender and the positive rate of gastric cancer patients in the expression of differences, and different Types of cancer, cell differentiation, metastasis of the depth of invasion of gastric cancer, gastric cancer Laurien stage and the relationship between the clinical stage, to studies the molecular mechanism of gastric cancer about the two factors in gastric cancer and precancerous lesions in the relationship between the exploration and gastric precancerous lesions.Methods: In this study we examined the expression of caudal-related homeo box transcription factor 2(CDX-2) with monoclonal antibody(CDX2-88)and LI-cadherin with Goat polyclonal antibody(C-17,sc-6987) using SP immunohistochemical detection of 246 cases of various stomach.Including two hundred and forty six specimens were collected,including normal epithelia(n=30), chronic superficial gastritis (n=30), chronic atrophic gastritis(n=42) , intestinal metaplasia (n=58),gastric adenocarcinoma(n=46), nearing cancer(n=30), gastric stromal tumor(n=10).The expression of CDX-2 was detected in alI the tissues by immunohischemistry.Results: The expression of CDX-2 in normaI epithelia and chronic gastritis are all negative , the positive rates of CDX-2 expresion in intestinal metaplasia and gastric adenocarcinoma are 91.4% and 80.4%,classification with Laurien, the positive rates of CDX-2 expresion in intestinal type and well-differentiated were higher than those in the diffuse type poorly differentiated (P<0.005).The expression of CDX-2 was independence of sex,age,depth of invasion, staging and lymph node metastasis. The nearing cancer and gastric stromal tumor exhibited negative CDX-2 expression.The expression of LI-cadherin in normaI epithelia and chronic gastritis are all negative , the positive rates of LI-cadherin expresion in intestinal metaplasia and gastric adenocarcinoma are 83 % (48/58) and 65 %(30/46),classification with Laurien, the positive rates of LI-cadherin expresion in intestinal type were higher(78%) than those in the diffuse type (35%) (P<0.050).LI-cadherin is positive correlation with lymph node metastasis and staging. The nearing cancer and gastric stromal tumor exhibited negative LI-cadherin expression. LI-cadherin expression closely related to the excessive of CDX-2 in gastric adenocarcinoma, forthemore, CDX-2 and LI-cadherin in the intestinal metaplasia gastric shown a strong relationship.Conclusion: The abnormal expression of CDX-2 was correlated with early stag of GC, maybe it plays an anti-oncogene role in the development of gastric carcinoma. GCs with high CDX-2 index have low invasion,high differentiation ,and Detection of CDX-2 expression in gastric cancer tisues can be useful in the determination of malignancy and prognosis.The abnormal expression of LI-cadherin was correlated with intestinal metaplasia and gastric adenocarcinoma. GCs with high LI-cadherin index have more lymph node metastasis, The Detection of LI-cadherin expression in gastric cancer tissues can be useful in the determination of malignancy and prognosis.CDX-2 and LI-intestinal cadherine are specific markers, and two in the normal physiological circumstances expression in the small intestine and colon mucosa. This study shows that in gastric intestinal metaplasia and gastric adenocarcinoma in the CDX-2 LI-cadherine regulating the expression level, the latter being induced by the former, especially in the early stages of cancer occur, and they may be playing an important role in tumor promoter . Local cancer violations or distant metastasis often lead to treatment failure of the main reasons, the incidence of gastric cancer on the molecular mechanism of the biological behavior and the search for new anti-transfer measures is crucial.