Experimental Research On The Use Of Mechanical Vibration And Compression Of Acupoints And Meridians To Treat Rat Cervical Nerve Root Injury

Objective:Using a self constructed mechanical vibration manipulation machine to treat rats with cervical nerve root injury using a treatment method of mechurgical methods.They were then allowed to heal for one week before the various treatment methods were performed.Afterwards,the rats were allocated randomly and equally into three groups that each contained forty eight rats.The first group was treated by manipulation of mechanical vibration and compression,the second group was treated by injecting NGF into the gluteus maximus major of the rats,and the third group which had received no medical treatment was the model comparianical vibration and steady compression of certain acupoints.The results were then analysed using nerve electrophysiology and immunohistochemistry to observe the effect of treatment methods on the recovery of damaged rat cervical nerve root tissue,and to speculate about the mechanism of recovery.Method:Right-sided cervical nerve root injury was induced in 144 Wistar rats by sson group.Each group was then allocated randomly and equally into four subgroups so that each subgroup contained twelve rats.These subgroups were named,7d(7 days),14d (14days),21d(21 days)and 28d(28 days).Each group was sacrificed at the appropriate time,and various parameters were taken.We measured and compared the diameter of both forelimbs,measured the electrophysiology of the cervical nerve root ex vivo,used immunohistochemistry to treat the submandibular gland,and observed the nerve cell ultrastructure under a microscope using HE-stained brachial plexus.Results:In the 14 day,21 day,and 28 day groups,the difference in forelimb diameter of the manipulation of mechanical group was significantly lower than that of the model comparison group(^a P＜0.05,^b P＜0.01).In the 21 day group,the difference in forelimb diameter in the manipulation of mechanical group was significantly lower than that in the NGF treatment group(^cP＜0.05).In the 28 day group,the difference in forelimb diameter in the NGF treatment group was significantly lower than that in the model comparison group (^aP＜0.05).In the 14 day,21 day and 28 day group,the MNCV of the manipulation of mechanical group was significantly faster than that of the model comparison group(^aP＜0.05,^b P＜0.01). In the 28 day group,the MNCV in the manipulation of mechanical group was significantly faster than that in the NGF treatment group(^c P＜0.05).In the 28 day group,the MNCV in the NGF treatment group was significantly faster than that of the model comparison group (^acP＜0.05).The MNAP in the 14 day,21 day,and 28 day groups of the manipulation of mechanical group and were significantly higher than that of the model comparison group(^a P＜0.05,^b P＜0.01).The MNAP of the manipulation of mechanical group was significantly higher than that of the NGF group in the 21 day and 28 day groups(^cP＜0.05).The MNAP of the manipulation of the NGF group was significantly higher than that of the model comparison group in the 21 day and 28 day groups(^aP＜0.05)In the 7day group,NGF concentration in the rat submandibular gland of the model comparison group was significantly higher than that of the manipulation of mechanical group and NGF group(^a P＜0.05).NGF concentration in the rat submandibular gland in the 21 day and 28 day groups of the manipulation of mechanical group and NGF group were significantly higher than that of the model comparison group(^a P＜0.05)Conclusion:This study show that mechanical vibration and steady compression of acupoints can effectively prevent muscle atrophy,increase the speed of injured right forelimb function recovery,induce submandibular gland NGF secretion,and induce the recovery of injured cervical nerves.