Research On The Mechanism Of Action Of Mechanical Vibration And Compression Of Meridians And Acupoints To Treat Rat Cervical Nerve Root In Jury

Objective:A self constructed manipulation machine is used to treat rat cervical nerve root injury by mechanical vibration.The effectiveness of the mechanical vibration treatment in treating cervical nerve root injury was observed and the mechanism of action of this treatment was elucidated.Method:150 Wistar rats were randomly separated into three groups.Each group contained 50 rats. The first group was called the mechanical manipulation group,the second group was the NGF group,and the third group was the model comparison group.Cervical nerve root injury was surgically induced in the right side of all the rats,and after 7 days of healing,each group was then further subdivided into five subgroups,each containing 10 rats.One subgroup from each main group was used to obtain original values before any treatment was performed,and the remaining four subgroups were named the 7 day,14 day,21 day,and 28 day subgroups.The mechanical manipulation group used a treatment method of mechanical manipulation using the self constructed manipulation machine,the NGF group used a treatment of intramuscular injection of NGF into the gluteus maximus of the rat,and the model comparison group received the same rearing treatment as the other groups,but received no medical treatment.Several parameters of the rats were taken at the appropriate times.These parameters included gait observation,grasp analysis,diameter measurements of both forelimbs,ex vivo electrophysiology on the rat right brachial plexus,and the submandibular gland was observed using immunohistochemistry and electron microscopy. These parameters were all taken from the "original value" subgroups of each subgroup on the first day of treatment and then each subsequent group was sacrificed at the appropriate times(7 days,14 days,21 days,and 28 days)and the parameters were also taken and recorded.Results:In the comparing the differences in the movements of the injured limb of the rats,there was significant difference(P＜0.05)between the different groups in the day 7 and 14 subgroups, and there was also significant difference(P＜0.05)when comparing the different treatment day subgroups within each treatment group.The mechanical manipulation group was significantly different(P＜0.025)from the NGF and model comparison groups on days 7 and 14,which means that mechanical manipulation is better at stimulating recovery of the injured limb than the other groups in the early stages of treatment.The difference in forelimb diameter was statistically significant between each treatment group and each treatment day subgroup.At the end of treatment,there was a statistically significant difference between the mechanical manipulation group and the model comparison group,demonstrating the effectiveness of mechanical manipulation in preventing and treating muscle atrophy.The MNAP values of the injured rat brachial plexus were statistically significant at different times and statistically significant in the different treatment groups in relation to time periods. After treatment was completed,the mechanical manipulation group was statistically different than the other two treatment groups,demonstrating the effectiveness of mechanical manipulation in stimulating the MNAP recovery of the injured brachial plexus.Statistical significance was found in MNCV values between the different treatment day subgroups. As time increased,the mechanical manipulation group showed a certain degree of recovery. The submandibular gland NGF average gray scale values were statistically significant between the different treatment groups,different treatment day subgroups,and treatment day subgroups in relation to treatment groups.The mechanical manipulation group values increased as time went on,reached a peak at 14 days,and then dropped afterwards.Conclusion:Mechanical manipulation treatment of rat cervical nerve root injury is an effective treatment method that has obvious results.This experiment provides a reliable method for future studies on the fundamentals of manipulation.At the same time,it provides scientific evidence on which clinical manipulation treatment can be used to treat nerve root injury related neck injuries.