Evaluation Of Peri-operative Peripheral And Renal Venous Levels Of PDGF-BB,VEGF And MMP-9 And Their Relevance In Patiens With Renal Cell Carcinoma

Objective: To evaluate peri-operative peripheral and renal venous plasma levels of plateletderived growth factor type BB (PDGF-BB), vascular endothelial growth factor (VEGF), and matrix metalloproteinase-9 (MMP-9) in relation to pathological variables, as pro-angiogenic factors are important for tumour growth and treatment of patients with renal cell carcinoma (RCC).Methods: The study included 29 consecutive patients with sporadic RCC who had tumour nephrectomy. Peripheral venous blood was drawn 24 hours before, and 48,96 hours after surgery. Renal venous blood was collected in a subgroup of 19 patients during surgery. The variables were analysed usingquantitative enzyme-linked immunoassay kits, and associated with pathological variables .Results: PDGF-BB(pg/ml),VEGF(pg/ml) and MMP-9 (μg/ml) of 24 hours before, 48 and 96 hours after surgery are 8419～15790(13750),7581～11476(9355), 8327～15674 (11829);356.3～1284.5(732.5),264.5～695.2(458.3),418.5～1209.3(624.1),and 403.3～954.7(677.2),250.7～582.7(384.6),374.7～886.4(651.4). This pro–angiogenic factors of renal venous are 10337～24757(18428), 396.4～1658.4(937.4) and 694.5～1484.3(1035.5).The level of serum VEGF with low differentiation neoplasm (G3) and late stage neoplasm(Ⅲ,Ⅳ)was higher than those with high differentiation (G1, G2) and early stage neoplasm(Ⅰ,Ⅱ).Conclusions: Soon after surgery, peripheral venous PDGF-BB, VEGF and MMP-9 levels were decreased.Renal venous PDGF-BB, VEGF and MMP-9 levels were higher than in the general venous blood pool,implying that the tumor-bearing kindey is the source of these factor. Early peripheral and renal venous VEGF levels were associated with pathological variables.