Design Of Dual-wavelength Laser Therapeutic Instrument And Effects Of The Function Of Macrophages Exposed To Low-energy Laser

ObjectiveVarious studies have confirmed their potential beneficial effects for wound healing and other types of tissue repair using both in vivo and in vitro settings. Evidence indicates that low-energy laser biostimulation can influence the behavior of many cell types, and that multiple effects can occur simultaneously. However, the exact mechanisms of biomodulation and biostimulation effects of laser irradiation are still far from understanding. Nevertheless, much more research is required to elucidate the exact mechanism of action of low-energy laser biotimulation at cellular and molecular levels. The biological effects of laser irradiation depend on wavelength, power, time of exposure and irradiation dose or fluence. So far, biostimulatory effects of low-energy laser irradiation on macrophage have been studied using He-Ne laser. The effect of 650nm and 532nm laser irradiation on macrophage has not been studied yet. On the basis of these considerations, the purpose of this article is to design a dual-wavelength semiconductor laser therapeutic instrument, and then to perform a research to evaluate the effect of 650nm and 532nm laser irradiation on mice peritoneal macrophage.Methods⑴The dual-wavelength semiconductor laser therapeutic instrument is controlled by AT89S51. The man-machine interface system can adjust output beam power and working hours;⑵Peritoneal cavity was douched using RPMI-1640 medium without FCS, and the contain cells were drawn out and were cultured in RPMI-1640 medium containing 10% FCS. The cells were identified by morphological observation, the survival rate of macrophage was calculated with trypan blue stain, the purity of macrophage was analyzed by Wright's staining;⑶Peritoneal macrophages of mice were irradiated with low-energy 650nm and 532nm semiconductor laser using various output power and working hours. The phagocytosis ability of peritoneal macrophages to the dye neutral red was measured;⑷Peritoneal macrophages of mice were irradiated with low-energy 650nm and 532nm semiconductor laser at the power of 10mW for 1,3,5,7 and 9 days respectively, 10 minutes each day. The activities of LDH and ACP were detected by chromometry.Results⑴The dual-wavelength semiconductor laser therapeutic instrument has dual-wavelength of 650nm and 532nm, we can adjust output beam power and working hours through the man-machine interface system;⑵The obtained cells were highly purified macrophages with morphologic characteristics of the macrophage in organism;⑶Low-energy 650nm and 532nm laser in certain dosage could significantly augment the peritoneal macrophage phagocytosis ability. The examination of the same power groups changed in similar trend along with time after irradiation. The phagocytosis ability after low-energy 532nm laser irradiation is similar to, but greater than that of low-energy 650 nm laser;⑷The activities of LDH and ACP were significantly increased after low-energy 650nm and 532nm laser irradiation. In addition, they firstly increases and then decreases with the increase of time, reaching maximal on day 7 and decreasing on day 9.Conclusion⑴The dual-wavelength semiconductor laser therapeutic instrument has dual-wavelength of 650nm and 532nm, we can adjust output beam power and working hours through the man-machine interface system. It has many advantages such as small, simple operated and so on, which can be used in clinical applications of laser physical therapy;⑵The obtained cells were highly purified macrophages with morphologic characteristics of the macrophage in organism. It's a simple and pragmatic method for separation and cultivation of mice peritoneal macrophages;⑶The present study suggested that low-energy 650 nm and 532nm laser in certain dosage could be capable of enhancing the phagocytosis ability and enzyme activities of mice peritoneal macrophages in vitro. Low-energy 650nm and 532nm laser irradiation can both stimulate peritoneal macrophages of mice. They could be capable of enhancing immune function of peritoneal macrophages of mice;⑷Standardized treatment parameters of the laser therapy should be followed and efforts should be made toward evaluating precise dosimetry for various medical conditions.