Study On The Effect Of Combination Of Artesunate With TNF Related Apoptosis Inducing Ligand (TRAIL, Apo-2L) On Prostate Carcinoma PC-3 Lines In Vitro

Objectives: To investigate the anti-proliferation, cell cycle arrest, induceing apoptosis and down regulate PSA effects and mechanism of Artesunate with TNF related apoptosis inducing ligand (TRAIL) on prostate carcinoma cell lines PC-3 in vitro, which may provide some clues to clinical application.Methods: (1)According to references and the results we studied before, we combinate four concentrations of Artesunate with three concentrations of TRAIL on human prostate carcinoma cell lines PC-3, the inhibition ratio of proliferation were investigated by MTT assay. (2)According to the results of MTT, three concentrations of Artesunate were conbimated with two concentrations of TRAIL on PC-3 cells, after treated 48hours, cell cycle were analyzed by flow cytometry(FCM) assay. (3)Three concentrations of Artesunate were conbimated with two concentrations of TRAIL on PC-3 cells, after treated 48hours, cell apoptosis ratio were investigated on flow cytometer by Annexin V-FITC/PI double staining method. (4)Three concentrations of Artesunate were conbimated with two concentrations of TRAIL on PC-3 cells, after treated 48hours, PSA of cell culture fluid were detected by ELISA method.Results: (1)MTT results showed that combination of Artesunate with TRAIL can effectively inhibit proliferation of human prostate carcinoma cell lines PC-3. Factorial analysis variance showed that Artesunate and TRAIL could inhibit proliferation of PC-3cells in dose dependent manner. (2)Cell cycle analysis results of FCM showed that after treated by combination of Artesunate with TRAIL 48hours, a concentration dependent increase of cells in G2/M phase could be detected, but no obvious change of cell count was found in G0 /G1 phase and S phase. (3)Cell apoptosis ratio were assayed on flow cytometer by Annexin V-FITC/PI double staining method, the results showed that combination of Artesunate with TRAIL can effectively induce PC-3 cells apoptosis, both of two has dose dependent manner. (4)ELISA results showed that a concentration dependent decrease of PSA of cell culture fluid were detected.Conclusions: Combination of chinese medicine Artesunate with TRAIL can actively inhibit proliferation of prostate carcinoma cell lines PC-3, induce cell cycle arrest at G2/M, effectively induce cell apoptosis of PC-3, and decrease the PSA level of cell culture fluid. All of these effects have well does dependent manner with Artesunate and TRAIL, factorial analysis variance showed that the function of simultaneous two agents was higher than only one agent applying. Chinese medicine Artesunate combinate with TRAIL will be a new therapeutic regimen in clinic therapy of prostate carcinoma.