The Experimental Research Of Buyanghuanwu Compounded Improving The Spinal Cord Ischemia-Reperfusion Injury

Goal: To investigate the effects of Buyanghuanwutang on spinal cord ischemic-reperfusion injury and discussion the mechanism.Method: SD rats 54, female and male, divides into 3 groups stochastically. A group (blank group) 18,B group (traditional Chinese medicine treatment group) 18, C group (control group) 18. The A group in tests previous 3 days to give Ding Anka physiological, 2/days, until take the marrow. The B group in tests previous 3 days to give Buyanghuanwutang, 2/days, until take the marrow. The C group in 30 minutes which pours into again in the ischemia injects the methyl loose dragon.The 3groups use the renal artery to block the law separately to create the spinal cord ischemia, 30 minutes later executes pours into again. The technique finishes, the injection celebrates magnamycin 10,000 IU to prevent the infection, is warm by the temperature measurement anus, maintains the animal body temperature at 38.5±0.5℃. Then after pours into again below (1, 2, 4, 8, 12, 24H) according to the design carries on separately: 1. executes the rats to take the spinal cord, ues the HPLC法makes the excitability amino acid the content examination. 2. The immunity group reduction examines in the rats spinal cord to perish weakly the gene (bax) expression situation. 3. Observes the rats hind legs nerve function change, and records nerve function grading ( NUROLOGIC DEFICIT SCORE, NDS) .Finally: Excitability amino acid test result demonstration, B group (traditional Chinese medicine treatment group)and C group (control group) excitability amino acid production quantity is lower than A group (blank group) obviously (p<0.01) ; Immunity group picture demonstration masculine cell number B group (traditional Chinese medicine treatment group)and C group (control group) is lower than A group (blank group) obviously, Namely perishes the gene (Bax) expression situation is weakly B, the C expression quantity is lower than A obviously (p<0.01) ; Nerve function grading (NDS) result demonstration, B group (traditional Chinese medicine treatment group)and C group (control group) is higher than A group (blank group) (p<0.01), Three group of C grading are highest. At the same time, demonstrated finally the big mouse spinal cord damage, in pours into latter 4, 8H to achieve the peak value again, the traditional Chinese medicine treatment group curative effect is ideal.Conclusion: The Buyanghuanwutang pours into the damage aspect in the prevention spinal cord ischemia to have the reliable curative effect again, in after the side Zhu Yaoneng reduces pours into the damage, again the excitability amino acid production effectively, reduces the excitability nerve toxicity, reduces the neuron to suffer injury; Reduces perishes weakly the gene expression, reduces cell weak perishing rate, thus causes the nerve function to receive the protection.