The Study Of Apoptosis In HepG2 Cells After Combined Transfection With LIGHT And IFN-γ

Objective:To investigate the expression of Fas,FasL and the apoptosis of liver cancer cell line HepG2 transfected with LIGHT and IFN-γgene mediated by Cationic liposome.Methods:Dividing HepG2 cells into the solo transfection of LIGHT gene,the combined transfection of LIGHT and IFN-γgenes and the controll,then transfect HepG2 cells mediated by Cationic liposome with pcDNA4C-LIGHT cDNA and pcDNA4/HisMax-hIFN-γcDNA extracted from E.coli JM-109 and cellecting HepG2 cells on 12h,24h and 48h after transfection,to investigate the apoptosis of HepG2 cells and the expression of Fas and FasL of HepG2 cells with flow cytometry.Results:After transfection,the expression of Fas and FasL increased in HepG2 cells,but the expression of FasL was lower than that of Fas.The expression of Fas in solo transfection of LIGHT gene group is higher than that in the controll group,and the combined transfection group of LIGHT and IFN-γgenes is highest(P＜0.01).The expression of FasL in the combined transfection of LIGHT and IFN-γwas higher than that in the solo transfection of LIGHT on 12h and 24h,but was lower than that in the solo transfection of LIGHT on 48h after transfection(P＜0.01 ).The apoptosis of HepG2 cells increased as time lasted,and the apoptosis of combined transfection of LIGHT and IFN-γwas higher than that of the solo transfection of LIGHT(P＜0.01).Conclusion:LIGHT and IFN-γgenes successfully transfected HepG2 cells mediated by Cationic liposome,and the expression of Fas and FasL on HepG2 cells was up-graduated and the apoptosis of HepG2 cells increasaed after transfection.The effect of the combined transfection of LIGHT and IFN-γgenes was better than that of the solo transfection of LIGHT gene.