Effects Of High Sugar Diet On Digestive System Free Radicals And Antioxidation Ability Of Mice

High sugar diet can cause glucose metablolism disorder and oxidative stress, leading to metabolic syndrome such as obesity, diabetes mellitus and cardiovascular diseases. While reactive oxygen special (ROS) is a determinant factor from glucose metablolism disorder to metabolic syndrome. This research is focused on following aspects: the effects on blood glucose and ROS level of intragastric(ig) high sugar mice; the effects of high sugar diet on digestive system ROS level and antioxidation ability of mice; the effects of lipoic acid(LA) and salvianolic acid B (Sal B) on digestive system ROS level and antioxidation ability of mice fed with high sugar diet. This research contributes to the pathology mechanism and regulation of chronic metabolic syndrome.80 Kunming mice were randomly divided into 8 groups. After a fast of 12 h, mice were administered respectively with saline, 20% glucose, 20% fructose, 20% galactose, 20% white sugar, 20% crystal sugar, 20% brown sugar, and 20% glucose + 0.002% Sal B. Blood glucose and the ROS level were determined at 0 h, 0.5 h, 1.0 h, 1.5 h, 2.0 h after administration. Another 6 groups of ten Kunming mice were fed with normal diet, high sugar diet (20% sucrose), high sugar diet with LA (20% sucrose + 0.05% LA), high sugar diet with LA (20% sucrose + 0.1% LA), high sugar diet with Sal B (20% sucrose + 0.002% Sal B) and high sugar diet with Sal B (20% sucrose + 0.004% Sal B), respectively. After 4 weeks, the ROS level and antioxidation ability of digestive system and blood were determined. Results were as follows: (1) ROS levels of ig monosaccharide mice: glucose > fructose > galactose; ROS levels of ig disaccharide mice: white sugar > brown sugar > crystal sugar; (2) High sugar diet led to increase of ROS level of blood and digestive system; the digestive system tissues'contributions to ROS level were changed: the contributions of liver and pancreas were significantly increased in which oxidative stress were most serious; (3) LA can scavenge ROS in blood and digestive system, increase the antioxidation ability of high sugar dietary mice, and the effect is better at a high concentration (0.1%); (4) 0.002% Sal B can lower the blood glucose, reduce ROS level in blood and digestive system, and significantly improve the antioxidation ability of mice fed with high sugar diet, while 0.004% Sal B can not.