Research Of The Protective Effect And Mechanism Of Salvianolic Acid B On Isolated Lung In Rats

Objective: To research and explore the protective effect of Salvianolic acid B on isolated donor.Methods:24SD rats were randomly divided into experiment group (A) and comparison group (B) with12rats each. Group B was perfused with raffinose-low potassium dextran (R-LPD). The perfusate used in group A had additional800mg/L of Salvianolic acid B compared with the perfusate used in group B. After removing the donor lungs with breathing machine working and perfusate perfusing, antegrade perfusion and retrograde perfusion made sure that donor lungs were thoroughly perfused. Then lungs were stored in4℃ice container with corresponding perfusion fluid surrounding. Wet/dry weight ratio(W/D), myeloperoxidase(MPO), malondialdehyde(MDA) and superoxide dismutase(SOD) were measured and pulmonary structure was observed by HE staining at different preservative periods of6h,9h,12h and24h after infusion.Results: For both groups, intragroup comparison:all parameters had no statistical difference before12h. However, W/D、MPO、MDA、SOD in24h were higher than those in12h (P<0.01). Interclass comparison:after4and8-hour preservation, all parameters of the two groups made no significant difference. Untill lung preservative period reaching24h, W/D, MDA and MPO were lower, but SOD was higher in group B than those in group A (P<0.05). Moreover, before12h preservation, the two groups did not show inflammatory injury, only manifested slight inflammatory reaction through histologic observation. Destructive pulmonary structure, alveolar interstitial edema and inflammatory damage were showed more clearly in group A than in group B.Conclusion:The perfusion that contained with Salvianolic acid B had no apparent superiority before12h. However, after12h, it could ensure the quality and prolong the preservation time of the donor lungs more effectively. Salvianolic acid B might play an important role in donor lungs protection.