Expression And Significance Of C-erbB-2 And VEGF In Colorectal Cancer Liver Metastases

Objective:To investigate the expression of c-erbB-2 and VEGF protein expression in liver metastases and its corresponding primary colorectal cancer,to probe whether there are any regularity of its express in liver metastases and its primary cancer and to analyze whether there are any relationships of their expressions with clinicopathologic characteristics.Also we make a survival analysis to know what make a negative affect on survival after hepatectomy of liver metastases.Methods:Forty-four patients operated between 2000 and 2007 in Shanghai Cancer Hospital for colorectal cancer liver metastases were included.Clinicopathologic data were obtained from the patients' records.Immunohistological EnVision staining was used to detect the expression of c-erbB-2 and VEGF in Forty-four formalin-fixed liver metastases and thirty-two primary colorectal tumors,and 15 cases of normal colorectal tissues and liver tissues at surgical margin were taken as control research respectively. SPSS13.0 was used to analyze the correlation between c-erbB-2 and VEGF expression and clinical pathological parameters,to probe whether there are any correlation and conformity between c-erbB-2 and VEGF expression in liver metastases and its primary.Results:1.The positive percentages of c-erbB-2 in liver metastases,liver paracancerous tissues,colorectal cancer,normal colorectal tissues were 38.64%,20.00%,31.25%,0.The c-erbB-2 expression levels were significance higher in colorectal cancer than its normal tissues(P＜0.05),but their difference were not observe between liver metastases and its paracancerous tissues(P＞0.05).The positive percentages of VEGF were52.27%,40.00%,40.63%,0%in liver metastases,liver paracancerous tissues,colorectal cancer,normal colorectal tissues.The VEGF were significance higher in colorectal cancer than its normal tissues (P＜0.05),but no difference were observe between liver metastases and its paracancerous tissues(P＞0.05).2.There is no significance different of c-erbB-2 and VEGF expression between liver metastases and primary cancer(P＞0.05).3.There is no significance correlation between c-erbB-2 and VEGF expression in liver metastases and primary cancer(P＞0.05).4.There is conformity of VEGF expression between liver metastases and its primary(P＜0.05),but not c-erbB-2(P＞0.05).5.The expression of c-erbB-2 was positively correlated with distributes and numbers of liver metastases(P＜0.05),but have no relationship with overall survival after hepatectomy of liver metastases(P＞0.05),VEGF expression was association with maximal diameter of liver metastases and poor survival (P＜0.05).Combine analyses of c-erbB-2 and VEGF may show better predictive role after resection of liver metastases.6.There is low strongly positive expression(++ and +++) of c-erbB-2 at liver metastases and colorectal cancer,which are 11.36%(5/44) and 9.73%(3/32) respectively,and mainly is cytoplasm positive.7.There is also overexpression of c-erbB-2 and VEGF at liver resection margin tissues,and sometimes there are higher expressions of VEGF at paracancerous tissues than tumor itself.8.Log rank test shows factors that VEGF expression have significance impact on overall survival after resection of liver metastases(P＜0.05),but Cox regression analyses didn't show it is an independent factors of survival(P＞0.05).Conclusion:1.C-erbB-2 and VEGF is common expressed in liver metastases and its primary cancer,c-erbB-2 and VEGF also are higher expression in liver resection margin tissuses,but not normal colorectal tissues.2.There is no significance different between c-erbB-2 and VEGF expression in liver metastases and primary cancer.3.There are no significance correlation between c-erbB-2 and VEGF expression in liver metastases and its primary cancer. 4.There is conformity of VEGF expression between liver metastases and its primary, we can roughly understand the liver expression of VEGF by check its primary colorectal cancer,but it is not the same of c-erbB-2.5.C-erbB-2 expression was positively correlated with distributes and numbers of liver metastases,but have no relationship with overall survival after hepatectomy of liver metastases,VEGF expression was association with maximal diameter of liver metastases and poor survival.Combine analyses of c-erbB-2 and VEGF may show better predictive role after resection of liver metastases.6.C-erbB-2 is mainly cytoplasm positive with low strongly positive.