Preparation And Study On Function Of Goat Placental Preparations

The goat placenta extracts were prepared by technological procedure and produced two kinds of goat placenta preparations(GPPⅠand GPPⅡ)which were respectively prepared by mixing the extracts of goat placentas with other kinds of supplementary materials according to two formulas designed.The amount of goat placenta extract in each capsule(net weight 500mg) of GPPⅠor GPPⅡis equivalent to 30g fresh placenta.The test of toxicity was based on the effects on immune function and hypoxia tolerance of the extracts and two kinds of different capsules of goat placenta were conducted in mice.These tests included acute toxicity test,30 days' treatment test in healthy condition,testicle chromosome aberration test,and myeloid cell micronucleus test. The immune function tests included lymphocyte transformation induced by concanavalin A and NK cytoactive of mice.The hypoxia tolerance tests included normal pressure hypoxia, sodium nitrite intoxation and acute cerebral ischemia.The mice in every group for each test for GPE,GPPⅠand GPPⅡwere fed one dosage to determine the optimal prescription of GPPⅡon the base of the former study. The mice were classed into three experiment groups and one control group randomly in GPPⅡ,There are 5 to 20 mice in every group according to the schedule.These mice were fed by artificial intragastric administration.The results are as follows:1.The results of acute toxicity test of GPE,GPPⅠand GPPⅡshowed that all treated mice were in good healthiness and no treated mouse presented abnormality or death,2.After treatment for 30 days,the mice in all experiment groups presented normal physiobehaviors,no toxic or death cases and no significant differences between the groups in utilization rate of food and hematology and blood biochemical indicators(P＞0.05),no pathological changes in the organs which were pathos copy,and no significant differences between the absolute weight and relative weight in the majority organs(P＞0.05),but absolute weight and relative weight of the spleen in group of GPPⅡwas significantly different to the index of control group(P＜0.05).3.Ames test showed that colony did not cause double increase of revertants at all doses in every bacterial strains no matter add the active system S-9 or not,and the dose-response relationship was not found.The results of Ames tests showed that GPE,GPPⅠand GPPⅡhad no mutagenic action.4.In the experiment of chromosome aberration in testicle cells of the mice experiment, the fragment and interchange amounts in all experiment groups' testicle chromosome and the chromosome(including euchromosome and sex chromosome) had the univalent number,the distortion cell number and the distortion factor had no significant differences compared with the negative control group(P＞0.05),but had significant differences from the positive control group treated CTX(P＜0.01).5.The micronuclear rates of the mice in all dosage groups had no significant differences from the negative control groups(P＞0.05),however,the micronuclear rates of the positive control group was significantly higher than the others(P＜0.01).6.The results of mouse blastisation of lymphocyte tests and NK cytoactive tests indicated that mice's splenic lymphocyte reproductive ability was significantly strengthened in GPPⅠand GPPⅡat single and middle dosage groups(P＜0.05),mice's splenic lymphocyte reproductive ability of GPPⅡwas low,middle dosage groups were most significantly(P＜0.01),but there were no significant difference in GPE group,and splenic lymphocyte reproductive ability of GPPⅡlow,high dosage groups were significantly different from the GPEⅠor GPE group(P＜0.05);NK cytoactive of GPPⅡsingle,low,middle dosage groups were significantly strengthened(P＜0.05),more over,the low dosage's difference was the most significant,while the others' were not significant(P＞0.05).7.The results of hypoxia tolerance tests indicated that the mice feed GPPⅡat single, middle,and high dosage groups' survival time were longer than that of the control group(P＜0.05),the other groups' survival time were not significantly longer.In the sodium nitrite toxic texts,the mice of GPE groups' survival time were significantly longer than the others(P＜0.05),and was significantly longer than the control group(P＜0.01),the mice of four groups' survival time in GPPⅠand GPPⅡgroups were significantly longer than the control group(P＜0.05),and the difference between groups were significant(P＜0.05);in Acute cerebral ischemia tests,the survival time of the mice of four groups in GPPⅡwere significantly longer than the control and GPPⅠ(P＜0.01),and the survival time of the mice of the high dosage group was significantly longer than GPE group(P＜0.01),and the difference between groups was also most significantly(P＜0.01).These results above indicated that GPPⅡcould strengthen the mice's hypoxia tolerance,and the function of GPPⅡwas significantly superior to GPPⅠand GPE.To sum up,GPE,GPPⅠand GPPⅡhad innoculty to the body,and had obvious favorable effects on the abilities of immunity and hypoxia tolerance.And GPPⅡwas the best among them.