Study On Rapid Propagation And Medicinal Components Of Bupleurum Chinese DC.

Thorowax is an important medical plant as well as popular crude drugs in China,South Corea and Japan.According to Chinese Medicine Dictionary published in 2005 AC,both Bupleurum chinese DC.and B.scorzonerifolium Wild.are two original plants for medical use.Roots of thorowax are used as medicine because they contain components such as saikoside and essential oil. These components are positive for health because of following effects such as anti-virus,anti-bacterial toxins,anti-inflammatory,anti-tumor, anti-convulsion,antipyretic,hypolipidemic,secretion promotion,liver protection,immunity improvement and etc.In recent years,the market demand of thorowax increases every year, but in contrast wildlife resources has been fewer and fewer due to over-exploitation.And the seeds of thorowax germination and cultivation techniques have difficult issues.Further more,there are different degrees of hybrid varieties phenomenon at almost every origin of Bupleurum,causing impure germplasm and uneven quality.The declined overall quality has affected the accuracy and stability of medicine quality.The export business of thorowax has also been affected unfavorably.The most urgent problem is producing thorowax plants with characteristics such as good quality,small variability and high-yield to supply market.The plants mentioned above should be gained by purifying and rejuvenating current popular artificial cultivation of germplasm.In order to mass-producing high quality seedlings and seeds of B. chinese DC.,1^st,we dispose seeds named Zhongchai No.1 through damaging the seed capsule or soaking them in different phytohormones;2^nd,applying the rapid propagation technology on B.chinese which are picked from eight different areas such as Linchuan,Wanrong,Zuoquan,Ruicheng,Linqiu, Fangshan county in Shanxi province,Longxi county in Gansu province and Shanglv county in Shaanxi province;3^rd,collected the B.chinese roots after being transplanted for six months,tested total saikoside by spectrophotometry, and determined the amount of saikosaponin a and d by HPLC-UV,tested if they meet quality standards;4^th,tested,compared and analysed the chlorophyll contents of thorowax leaves sampled from test-tube plantlets, transplanted plants grown in nutrition pot,transplanted plants grown in fields in the second year,plants grown in field by spectrophotometry.The experiment results in following conclusions:The phytohormone has little effect on seed germination of B.chinese,but scraping the testa could increase the germination percentage of seeds by 20%as well as shorten the germinating time greatly.The optimum medium for bud propagation was B₅ supplemented with 1.0mg/L 6-BA and 0.2mg/L KT.The optimum medium for root induction of test-tube plantlets was 1/2 MS added with 0.1mg/L NAA,0.5mg/L IBA and 1.0mg/L DSC.The annual propagation coefficient of B.chinese plants is more than 1×10⁸,and the survival rate of transplantation reaches 94%～97%.After transplanted in field for more than six months,the saikosaponin content of thorowax root meets the quality inspection standards. There are sufficient nutriments in bud stage and transplanting stage,growing condition for test-tube plantlets is good.After transplanted in nutrition pots, the photosynthesis ability has enhanced.