The Effect Of Jinke On Growth Transfer And Apoptosis Of Transplantable Tumor Model In Nude Mice With Breast Cancer

Objective To investigate the effects of Jinke on the tumour growth, cell proliferation and apoptosis in transplantable tumor of breast cancer cell line MCF-7 in nude mice.Methods MCF-7 cells were inoculated into the mammary fatty pad of nude mice to establish breast cancer model, then thirty BALB/c nude mice with xenograft tumor were randomized into two groups: Control group and Jinke group. The Jinke group was given Jinke while control one was given normal saline water by gastrogavage in 0.2ml volume for 30 days. The effects of Jinke on the tumor growth, the tumour volume and survival days were observed and recorded. Samples of tumor were collected. The sections of tumor were observed under light microscope. Cell apoptosis in situ was examined by a Tunel assay. The morphological variation of apoptosis was observed through immunohisto-chemical staining method.Results 1 The tumor volume of Control group were significantly larger than that of Jinke group(4.62±1.86 cm3和6.35±2.73 cm3,P <0.01). 2 The survival days of Jinke group were significantly longer than in Control group (116.7±28.5d和95.3±26.7d,P<0.01). 3 Detection of apoptosis in situ showed that apoptosis index (AI) was significantly higher in Jinke group than in Control group(19.32%和8.36%,P<0.01). 4 The flow cytometry showed that apoptosis was significantly higher in Jinke group than in Control group(18.21%和8.39%,P<0.01).Conclusion The Jinke could inhibit the growth of transplantation tumor of breast cancer cell line MCF-7 in nude mice, and extend the survival days.