Analytical Studies Of T.capillipes Gagnep. And 'Shuangdan' Decotion By Capillary Electrophoresis

Capillary electrophoresis (CE), as an important analytical separation technique, offers the advantages of excellent separation efficiency, high resolution and rapid analysis. In addition, the amount of sample, solvent consumption and hazardous waste solution produced by CE is at a minimum. Traditional Chinese medicines (TCM) are usually in the form of multicomponent prescriptions and each component may exhibit a complicated profile of constituents, so the substance system of TCM is extraordinary complex. It's worthwhile to explore CE technology used for the identification and determination of TCM as a research direction.This thesis set T. capillipes Gagnep. and 'Shuangdan' decotion as objectives and developed a series of correlative analytical studies of TCM by CE. The main contents are described as follows:Firstly, the analysis methods of TCM with non-aqueous capillary electrophoresis (NACE) have been studied in the sake of the reduced joule hot, the improved separation selectivity and separation efficiency. A NACE method for the separation and determination of palmatine and jatrorrhizine in T. capillipes Gagnep. from different geographic origins was established, it also can be applied to the analysis of other Chinese herbs containing these alkaloids, such as Rhizoma Coptidis, Cortex Phellodendri and Corydalis tuber.Secondly, it's difficult to separate the components contained in TCM and its preparations for the presence of ionic substances and neutral species. Milcellar electrokinetic capillary chromatography (MEKC) not only provides added selectivity in the separation of ionic substances, but also allows good separation of neutral species. An easy, simple and rapid MEKC method was developed for the separation and determination of protocatechuic aldehyde, danshensu, salvianolic acid B and gallic acid in 'Shuangdan' granules.Thirdly, A high performance liquid chromatography (HPLC) method with multiple detection wavelengths was used for the determination of gallic acid, paeonoland paeoniflorin contained in Cortex Moutan.Finally, the validation characteristics of MEKC were compared with those of HPLC during the analysis of paeonol and paeoniflorin. As a result, no significant difference was found between the two methods.In summary, the results of this thesis show that the performance of CE in TCM analysis is similar to that of HPLC. Thus, the study and application of CE should be emphasized for its rapid analysis, minimum amount of sample and solvent consumption.