| Sporothrix disease caused by Sporothrix schenckii give rise to deep fungal disease, which can lead to skin and subcutaneous tissue infections, rarely involving the visceral organs, systemic infection can be initially accompanied by inhalation or skin infection caused by spores. In recent years, a number of factors make deep or systemic disseminated infections continuously increasing. These factors include the irregular application of broad-spectrum antibiotics and immunosuppressive drugs, growing cancer patients with radiotherapy and chemotherapy treatment as well as AIDS patients, and so on..Sporothrix infection can cause skin, lymphatics ,skin tissues and other organs involved. The clinical symptoms is mainly associated with the host's immune status, also with strain virulence and infection pathway. In recent years, studies have shown that the immune mechanism of Sporothrix infection is mainly related to cellular immunity by activation of T cell and macrophages, while NO is the main product released from macrophages . Study shows that NO are defensive factor against Sporothrix . it is ofent seen that the NO values develop dynamic change in a lot of infectious diseases. So the changes in NO concentration can indirectly reflect the post-infection immune mechanism progress status.Objective:Through dynamic monitoring of serum NO content, indirectly reflects the immune status in the mice after infection, and calrify the relevance between the immune status at the same the case and toxicity of strains .then the implication of cultivated time on tocicolity of strains can be elucidated. This can provide diagnosic and therapeutic significance through the above-mentioned clinical studies on Sporothrix disease, and the application of adjuvant therapy on NO can be further studied.Methods:(1) Bacterium production, animal groups, specimen collection①three certified Sporothrix schenckii isolated were incubated in Sabouraud glucose peptone agar solid medium at 25°C, for one week or 2 weeks, respectively , 1×108CFU/ml concentrations of bacteria suspensions were made.②each mice was given CTX (CTX) 200mg/kg by intraperitoneal injection before 12-week-old BALB / C mice inoculated three days were infected by the bacterium. The 126 rats were randomly divided into into 7 groups. A-F group were injected 0.2ml bacilli by intraperitoneal injection. Control group were give equal 0.9% saline solution. group A: Vaccinate fixed Sporothrix schenckii strain one within 14 days. group B: vaccinate fixed Sporothrix schenckii strains one cultivated seven days. Group C: Vaccinate fixed Sporothrix schenckii strains 2 cultivated 14 days. Group D: Vaccinate fixed Sporothrix schenckii strains 2 cultivated 7 days. Group E: Vaccinate lymphatic type Sporothrix schenckii strains cultured 14 days. Group F: Vaccinate lymphatic type Sporothrix schenckii strains cultured 7 days. Control group: given equal volume of 0.9% sodium chloride injection.③randomly selected 6 mice at 1,2,3 weeks respectively in each group ,then 0.2-0.6ml of post-orbital venous blood was taken to anticoagulant EP tube, 1000rpm centrifuge eight minutes, the supernatant was taken from the application of NO measured kit. the mice were killed and dissected to observe the abdominal cavity and organs after blood, The organization from lesions then do fungal culture and histopathological examination.(2) all the data were deal with using SPSS15.0 software package for statistical analysis. Measurement data were presented with mean±standard deviation (±S) . n represent as the sample size, comparison between the three groups were analyzed using one-way ANOVA, two to two comparison is done by LSD-t test. P <0.05 or P <0.01 were considered to be statistically significance.Results:(1) there are no death and weight change in Sporothrix schenckii immunosu- ppressive mice, and the highest incidence are genital organs.the size of spleen is positively proportionally to incubation time There was a significant difference (P <0.05) in the change of spleen size in a three-week spleen Change. Lesions in mice infected with the fungal culture have seen atypical colony, its shape and characteristics was consistent with the clony cultivated. Histop- athology of Sporothrix is also in line with the clinicopathologic features.(2) the mice of immune suppression injected different Sporothrix schenckii strains by intraperitoneal and for various culture time, the serum NO content was significantly higher than (P <0.01)control group;thre are no significant difference between A group and C group, B group and D group which are cultivated for the same period (P> 0.05); All values in lymphatic type E group was significantly higher than the fixed type A group and C group with the same cultivated time. F group was higher than B group and D group (P <0.05); A group and B group, C group and D group, E group and F group were inoculated with different strains bacteria suspension of the same time to cultivate, the measured value with 7 days cultivated is higher than the corresponding cultivated 14 days of each group (P <0.05).Conclusions:(1)the weight changes of the mice after infection in Sporothrix schenckii during the whole experiment(2)infected mice significantly increased the weight of spleen which showed important defensive role of spleen;(3)serum NO can be used as a biochemical indicator to monitor Sporothrix schenckii infection;(4)at the same immune status and the same cultivate time, the serum NO content has a positive correlation with toxicity of strains .Thus pathogenicity of Sporothrix schenckii strains can be measured when there is equal immune status.(5)pathogenicity is related with strains of the same size and cultivating time, and pathogenicity of strains cultivating seven days are higher than strains cultivating 14-day.
|
PDF Full Text Request