| Sporotrichosis is a common fungal disease of the skin, chronicgranulomatous disease that is infected by Sporothrix schenckii. It can beclassified clinically into the following classifications: fixed pattern,lymphatics, disseminated histoplasmosis skin，and Disseminated type.The first two classifications are more common. Sporothrix schenckii is abipolar fungi. If it internally parasites in humans or in the37-degreeconditions, produces a yeast phase and at room temperature producedhyphal, yeast is the pathogenic bacteria spores, and sporothrix schenckiispores from hyphal bacteria in the body’s transformation is a key steps ofpathogenic yeast. Proliferation of yeast can be infected, forming smallspores pathogenic sprouting. The yeast can cause skin, subcutaneoustissue, mucous membranes, and lymphatic system, or even the wholebody system of local damage. However, hyphal won’t have harm, Thisbipolar pathogenicity of the fungus and its bipolar conversions are closelyrelated. Current research reports on the transformation of yeast genes tendto concentrate on the level of, and pathogenicity of less closely related tothe same physical, chemical and biological characteristics. Chinesescholar, Zhang Jing. Clear effects of temperature and culture medium canbe experimentally spore bacteria yeast transformation; Conditionalpathogens Candida albicans has a similar conversion, Chinese scholar,Liu Zehu. By Candida albicans in vitro Mycelial morphology, changingthe PH of the culture medium concludes that acidic environment for Candida albicans yeast formation, alkaline environment will besupportive of the mycelium formed. Partial alkaline environmentconducive to white Candida bacteria pathogenic, currently, for PH onspores silk bacteria yeast phase pathogenic of effects still no research,based on above theory, this research through discussion different PH inspores silk bacteria yeast phase into in the of role, clear has PH is effectsspores silk bacteria yeast phase into of key factors, for inhibition sporessilk bacteria in body of yeast phase into, blocking its pathogenicmechanism provides has research based. While exploring the growth ofspores of bacteria and yeast into the most optimum PH value range forPH value range, laying the Foundation for future clinical training andresearch.Objective:1. Compare the growth of sporothrix schenckii under different PHconditions. Explore the suitable pH values for sporothrix schenckii’sgrowth.2. Preliminary study the different pH values’ effect on the transitionbetween sporothrix schenckii and yeast, and discuss that the key factor onthe transition between sporothrix schenckii and yeast is pH value.Methods:1. Inoculate sporothrix schenckii in the sand castle (SDA) mediawhere the PH value is1-14. Put the sand castle media to the constanttemperature incubators that the culture is25℃for3weeks.2. Divide the sporothrix schenckii bacterial colony that has beenvaccinated in the general SDA and activated at25℃into groups andinoculate them in the brain heart infusion agar (BHI) medium with pH5,6,7,8, and9separately. Each group contains20specimens with culture 37℃, observe the growth of the colony and calculate the conversion rateof yeast cells.Results:1. The sporothrix schenckii inoculated in pH1-3medium does notgrow; grows not well in pH4,10,11,12,13, and14medium, grows wellin pH5-9medium. So the suitable pH range for sporothrix schenckii’sgrowth is pH5-9. Thus, set the medium with pH5-9for next yeasttransition test.2. All yeast cells are transited in BHI medium with pH=5, pH=6andpH=7;16specimens are transited in pH=8medium; No transition inpH=9medium. When calculating the sporothrix schenckii conversion ratein different PH medium using light microscopic, we found that theconversion rate in pH7and pH6are higher than that in PH5and pH8.The conversion rate of yeast cells in the pH=7media is up to90%; theconversion rate is lowest in pH=5and PH=8medium, with the differencestatistically significant (P<0.001). The conversion time and speed are notsame in different pH medium. Colony appears on the second day in pH=5media; grows fast in the third and fourth days; grows slowly after the fifthday. Colony appears on the third day in pH=6and pH=7media; growsfast in the fourth and fifth days; grows slowly after the sixth day. Colonyappears on the fifth day in pH=8media; grows fast in the sixth to eighthdays; grows slowly after the ninth day.Conclusions:1. Sporothrix schenckii grows well on the medium with pH5-9;grows not well with pH4,10,11,12,13, and14medium; hardly growswith pH1,2,3medium. Thus, the suitable pH range for sporothrixschenckii’ growth is pH5-9. 2. pH value is one of the factors that affect the transition betweenyeast and sporothrix schenckii. The transition can happen with pH5-8;the conversion rate is high with PH7. Thus, the suitable pH values for theyeast cells transition are pH7. pH5media for yeast transition occurredearlier, pH8media for yeast transition occurred later. The conversionrates in pH5and pH8are significantly lower than that with pH=6andpH=7. |
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