| In the thesis,the chemical constituents and bioactivities about the liquid supplements of fresh panax Ginseng refined with honey were studied for the first time. The liquid supplements contain eight valuable Chinese herbals,such as fresh Panax ginseng C.A.Meyer,Soybean isoflavone,Lycium barbarum L.,Ganoderma lucidum et al. There are many activive compounds,including steroid hormone,Saponins,alkaloids,flavonoids,polysaccharide,protein and inorganic elements,which have many hygienical functions.The polysaccharide constituent of the liquid supplements of fresh panax Ginseng refined with honey was studied for the first time. Six kind of polysaccharides were separated and purified by the method of water extraction and alcohol deposit, then the protein was removed by the method of Sevage and papain hydrolysis. Last,the pigment was eliminated by H2O2 oxidation, micromolecule and mineral salt were taken off by gel chromatography. The six polysaccharides were named as GPS-I,GPS-II,GPS-III,GPS-IV,GPS-V,GPS-VI whose molecular weight is 56302,30372,10601,10364,9247,4667seperately.On the other hand ,the other chemical constituents of the liquid supplements of fresh panax Ginseng refined with honey were investigated through the separation and purification by column chromatography of silica gel,eversed phase silica column, semi-preparative high performance liquid chromatography and recrystallization methods. 7 kinds of compounds were identified according to their physical-chemical properties and spectrum analysis including Sucrose(1),β-D-glucopyranosyl B glycoside(2), 4,2,4-trihydroxy chalcone(3), 7, 4-dihydroxy- flavanone-7-O-β-D-glucoside(4),4,2',4'-trihydroxy chalcone-4-O-β-glucopyranoside(5), pheny- lacetic acid(6), 20 (S) - ginsenoside Rh2(7), 20 (S) - ginsenoside Rh1(8).The total polysaccharide separated from the liquid supplements of fresh panax ginseng refined with honey is studied for the first time on anti-aging the classical D-galactose-induced mice aging model experiment,the animals were administrated with the total polysaccharide for 6 weeks in high,moderate and low three doses respectively,and the four biochemical parameters of aging were measured.The results showed that continuous subcutaneous injection of mice 6 weeks to D-galactose, measured in model group mice in the liver and plasma SOD decreased, MDA value increased; renal tissue GSH-PX dynamism and vitality of NOS have declined.Compared with normal group, model group mice aging of plasma superoxide dismutase significantly reduced viability (P<0.05), MDA content increased significantly (P<0.01). Superoxide dismutase indirectly reduce the dynamic response of the body's ability to clear the drop in oxygen free radicals, and the MDA content increased, indicating that the body to deepen the level of lipid peroxidation, which combination of the two biochemical markers, reflecting the emergence of the body the signs of aging on this experiment with D-galactose-made model of successful aging.Compared with model group, the polysaccharide of the liquid supplements of fresh panax ginseng L. refined with Honey (GSPS)25mg ? kg-1 ? d-1, 50mg ? kg-1 ? d-1 dose group significantly increased superoxide dismutase vitality (P <0.05) to enhance the body of oxygen free radicals on the inhibition rate was significantly reduced in liver tissue and plasma MDA levels (P<0.05). Lian fresh ginseng honey Polysaccharide rehydration 50mg ? kg-1 ? d-1 group significantly increased superoxide dismutase glutathione peroxidase vitality (P <0.05). Lian fresh ginseng and honey Polysaccharide rehydration high, medium and low dose group of the three NOS activity are not clear. Description of fresh ginseng honey Lian Polysaccharide rehydration has a good anti-aging effect, which may be related with the removal of free radicals. Pharmacological study shows that total polysaccharide can enhance and strengthen the body's ability of anti-senescing.
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