The Apoptosis And Expression Of Survivin And Caspase-3 In HepG₂ Cells Transfected With LIGHT And IFN-γ

Objective To transfect the LIGHT and IFN-γinto HepG₂ cells mediated by cationie liposome and detect cell apoptosis and the expression of Survivin and Caspase-3 in transfered HepG₂ cells. Methods The pcDNA4C-LIGHT-cDNA and pcDNA4/ HisMax-hIFN-γextracted from E.coli JM-109, were transfected into HepG₂ cells mediated by cationie liposome. The HepG₂ cells was collected after transfection of 12h, 24h, 48h respectively. The cell apoptosis and the expression of Survivin and Caspase-3 was detected by flow cytometer(FCM). Results The cell apoptosis and expression of caspase-3 increased by degrees among control group, LIGHT-mediated group and cotransfection group, the expression of Survivin decreased by degrees. Conclusion LIGHT had an effection on inducing the apoptosis of HepG2 cells by regulating the expression of caspase-3 and Survivin, probably. IFN-γcan sensitize its effection significantlly.