Effects Of Dry Matter Of Culture Broth Of Inonotus Oblique And Its Components On The Improvement Of Pathological Changes In Diabetes Mice

Inonotus obliquus (Fr.) Pilat has been known for over one hundred years as a medicinal fungus in folk medicine, which can treat many refractory diseases. However, this fungus is just investigated in China at present though being explored extensively in American, Korea and Japan. Since American Humfeld H and other workers studied submerged culture of mushroom and put forward to culture mycelia of mushroom by this method, fungi have been cultured in succession. But the researches about I. obliquus are poor in this aspect. Until now it has not been studied chemically, manipulately, pharmacologically and so on.This investigation characterized the chemical composition of dry matter of culture broth (DMCB) of I. obliquus in submerged culture, including moisture, ash, fat, crude protein, amino acid, triterpenoids, total phenol, polysaccharrides, reducing sugar and minerals. It's shown that the DMCB of I. obliquus in submerged culture was rich of crude protein, amino acid and polysaccharides, the contents of which were 25.73%, 14.50% and 28.55%, respectively. And unsaturated fatty acids were prior to saturated fatty acids, in which oleic acid and linoleic acid were the main components. There were also many other nutrition components in it. Moreover, the content of As and Pb were 0.50 and 0.30μg/g, respectively, which met national standards. The monosaccharide components of polysaccharides extracted under the optimum condition were arabinose 0.53%, mannose 0.48%, glucose 10.75% and galactose 2.44%. Furthermore, there were acidic polysaccharides in the products and the glycosidic bonds wereα-types mainly. Simultaneously, an extraction temperature of 83℃, an extraction time of 2.2 h and a liquid-solid ratio of 33.3 were found to be optimal for polysaccharides extraction from DMCB of I. obliquus. By means of additional experiments, the adequacy of this model was conformed.The antihyperglycemic and antilipidperoxidative effects of the DMCB of I. obliquus and its extractions were investigated in normal, glucose-induced hyperglycemic and alloxan-induced diabetic mice and the possible mechanism was also discussed. Treatment with the DMCB of I. obliquus and its extractions exhibited a mild hypoglycemic effect in normal mice, and failed to reduce the peak glucose levels after glucose administration. However, euglycemia was achieved in the DMCB of I. obliquus and its extractions treated mice after 120 min of glucose loading. In alloxan-induced diabetic mice, the DMCB of I. obliquus and its extractions showed a significant decrease in blood glucose level. Furthermore, the DMCB of I. obliquus and its extractions treatment significantly decreased serum contents of FFA, TC, TG and LDL-C, whereas effectively increased HDL-C, insulin level and hepatic glycogen contents in liver on diabetic mice. Besides, the DMCB of I. obliquus and its extractions treatment significantly increased CAT, SOD and GPx activity except for decreasing MDA level in diabetic mice. Histological morphology examination showed that the DMCB of I. obliquus restored the damage of pancreas tissues in mice with diabetes mellitus. The results revealed that the DMCB of I. obliquus and fraction II of DMCB possessed of significantly antihyperglycemic, antilipidperoxidative and antioxidant effects in alloxan induced diabetic mice.