| Platelets can protect vessel wall and is very useful for blood clotting. Platelet concentrates(PCs) infusion is necessary to the patients of massive blood loss and thrombopenia induced by chemotherapy and radiotherapy. Adequate platelets supply is a limited step to clinical therapy. At present,there have no effective succedanea of platelets and platelets can only be collected from donors. The clinical requirement for platelets is increasing rapidly because platelet transfusion has been an important measure for hemorrhage with thrombocytopenia, but the clinical use of fresh platelet concentrates is limited due to insufficient resources, short shelf life at room temperature and high contamination risk. Storing platelets at 22℃is good to maintain function of platelets,in vivo recovery and survival. However,platelets stored at 22℃is facilitate to bacterial contamination and can not reach the requirement of clinic for its 5 days storage. Storing platelets at 4℃is now known to reduce metabolic activity, alter the morphology from discoid to spherical and decrease the responses in various in vitro tests.Many cytokines (such as TGF-β,IL-1βand IL-8) contained in plasma may cause some transfusion reactions. Platelets preservation research is hotspot and difficulty in transfusion medicine. The storage of platelets (PLTs) in PLT additive solutions (PASs) can reduce allergic and febrile transfusion reactions,facilitate ABO-incompatible PLT transfusions,enable pathogen inactivation,and make more plasma available for other purposes. For this reason,there has been considerable focus on the development of new PASs that assure maintenance of good PLT quality throughout storage.Our study was designed to added trehalose into PLT additive solutions (PASs) and modified PLT additive solution(PAS-ⅢM) was used to be a substitute for plasma to maintain the function of the platelets stored in relative cold (10℃) temperature.Objective To evaluate the in vitro and in vivo efficacy of modified platelet (PLT) additive solution(PAS-ⅢM) with trehalose as a substitute for plasma for the storage of platelet concentrates at low temperature (10°C).Methods Apheresis platelets from 6 donors were divided and stored in different media(A: 100%plasma, B: 70%PAS-ⅢM/30%plasma, C:100%plasma/trehalose), and each media group stored at 10℃, 22℃and -85℃respectively. The samples of each platelets were respectively collected on day 0,day 1, day 5, day 7 and day 9 after storage (samples of platelets stored at -85℃were collected on day 20 after storage) and platelet count(PLT), mean platelet volume(MPV), platelet distributing width(PDW), CD62p, hypotonic shock response(HSR), platelet aggregation, pH, lactic dehydrogenase(LDH), glucose and morphology of platelets were evaluated. Rabbit platelet concentrates were collected and stored in different media and temperature conditions, plasma (22°C), 70%PAS-ⅢM/30% plasma (10°C) and plasma/trehalose (-85°C). The platelets of plasma media (22°C) on storage day 3 and 70%PAS-ⅢM/30% plasma (10°C) on storage day 3, 7, 9 and plasma/trehalose (-85°C) thawed on day 20 were used to transfuse into rabbit model. The in vivo recoveries and survivals of the three preserved platelets and fresh platelets as control were measured and evaluated after transfused into animal model of rabbit thrombocytopenia.Results The expression of CD62p of A group and B group were increased in a time-dependent manner, but HSR and platelet aggregation were decreased. The expression of CD62p, LDH release, GLU consumption, platelet aggregation of A group were significant higher than that of B group(P<0.05). HSR of A group were significant lower than that of B group(P<0.05). After 5 days, pH of B group were significant decreased(P<0.05). LDH release were significant high in samples of C group and the expression of CD62p were lower than that of other two groups(P<0.05). PDW of C group were increased after storage and significant higher than that of other two groups(besides that of B group on day 5) (P<0.05). In all storage groups, PLT were no significant difference(P>0.05). It is concluded that the protective effects of 70%PAS-ⅢM/30%plasma (10°C) and plasma platelets (22°C) on morphology of platelets are similar, but better than that of plasma platelets (4°C) and plasma/trehalose (-85°C).Besides the survival of platelets of 70%PAS-ⅢM/30%plasma(10°C) on day 9 was lower than that of fresh platelets (P<0.05), recoveries and survivals of plasma platelets (22°C), 70%PAS-ⅢM/30% plasma platelets (10°C) and fresh platelets (22°C) were no significant difference(P>0.05). Recovery and survival of frozen platelets were significant lower than that of the other groups (P<0.05).Conclusion PAS-ⅢM with trehalose could be a good substitute for plasma for platelet storage,and can protect the chilled platelets,prolong the circulation of refrigerated platelets.
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