The Function Of Galactosylation Rabbit Platelet In Vivo And In Vitro

Objective: This study was aimed to investigate the function of galactosylation cooled rabbit platelet in vivo and in vitro.Methods: We collected rabbit heart blood, prepared concentrated platelet suspension in a normal way to which we add UDP-Gal ,and then stored them for ten days in 4℃refrigerator. Thereafter,platelet count,mean platelet volume ,platelet distributing width ,platelet aggregation function,platelet activity to urge coagulation includingPF3aT and APCT and apoptosis were determined .Meanwhile,survival time in vivo was tested after cold-stored rabbit platelets labeled with Cr51 were transfused into rabbits; Rabbit ear bleeding time ,APCT, percentage plate recovery(PPR) were determined in the first and the twenty-fourth hour after they were transfused into model of rabbit thrombocytopenia.Results: There is not significant difference for PLT count,MPV,PDW,PF3aT and APCT between UDP-Gal cold-stored platelet group and fresh platelet group(p﹥0.05).On the contrary,platelet count decrease significantly ,MPV,PDW jump and PF3aT and APCT go down in cold contral group compared with fresh platelet group(p﹤0.01).Apoptosis increase in UDP-Gal cold-stored platelet group compared with fresh platelet group(p﹤0.05),but is significantly lower than that in cold contral group(p﹤0.01). Although PagT(inducing reagent:C-PG) decrease,it can still be above 50% of fresh platelet;Survival time in rabbit vivo is close between UDP-Gal cold-stored platelet group and fresh platelet group(p﹤0.05). Survival rate in the seventy-two hour after transfusion in fresh platelet group, UDP-Gal cold-stored platelet group and cold contral group respectively is 57.5%±7.2%,50.3%±6.3%和0.1%±0.5%; Rabbit ear bleeding time was significantly shortened after transfusion of galactosylation cooled rabbit platelet,and there was not significant difference between UDP-Gal cold-stored platelet group and fresh platelet group(p﹥0.05).On the contrary,it had less change in cold control group(p﹤0.01);PPR was respectively 66.1%±0.5%,47.8%±0.6%;60.9%±0.3%,41.6%±0.4%;47.7%±0.5%,11.4%±0.5% in fresh platelet group, UDP-Gal cold-stored platelet group and cold control group. PPR after transfusion of galactosylation cooled rabbit platelet has no statistical difference compared with that of fresh platelet group(p﹥0.05).Conclusions:Galactosylation can effectively enhance the function of cooled platelet in vivo and in vitro, and prolong the storage time of them.