Pharmacogonostical Study On PiPaHua(Flos Eriobotryae)

In the traditional application,foliage is the effective part of Eriobotryae,which was first reported in as a normal material.From then on the following pharmacopoeia all have adopted this material,until now the has adopted Eriobotrya japonica(Thunb) Lindl.as the official specie of this material.In folk application,people use the flowers as the effective part in,this use first appeared in the ,"PiPaHua can mainly treat the headache and the cold."During the maturing period,among all the flowers,there are only 5-10%can finally give birth to fruit,and the rest will waste lots of the nutrient, then the farmers have to remove the extra flowers to guarantee the harvest of the fruit. In order to take good use of the extra flowers,we design a systematic research plan to study the pharmacognosy of PiPaHua including the morph-histology, physical-chemical characters identification method,content assay method and the pharmacological effects.The results indicated:in morph identification:the appearance,color,clothing hair and the flavour are the main identification characters;in micro identification:the light yellow round or blunt triangle pollen grain and the nonglandular hair are the main identification characters;in the chemical research:the preliminary tests suggested the flowers contained triterpenoid saponin,organic acid,polysaccharide,etc.we took ursolic acid as the control compound establishing the TLC spectrum identification method,then we tested the extract content of 9 batches samples collected from different area,the results indicated the extract of the flowers which collected during the blossom period have the highest quantity;sequently,we determined the ursolic acid content in flowers by means of HPLC for the first time,the results showed that the content of ursolic acid in Sichuan produced sample was more than0.3%,which was higher than the Guangxi produced one.And the content of ursolic acid is higher in flowers than in bud.In the pharmacological research,the acute toxicity experiment results suggested the flowers are safe material and the pharmacological experiments on antitussive,expectoration and antiinflammation effects suggested an efficient bioactivity effect.This experiment has done the pharmacognosy research on the flowers of Eriobotrya japonica(Thunb) Lindl.,which provided the basic foundation for broaden the medicinal part,promoting the practical exploration of PiPaHua.Attached 4 pictures for plant,raw material,histology and powder identification, 12 graphs for chromatography.