| ObjectiveTo investigate the genetic instabilities of bone marrow hematopoietic cells from patients with aplastic anemia and evaluate the impact of IST in aplastic anemia evolving into clonal hematologic disorders.MethodsComet assay were used to evaluate the genetic instabilities of hematopoietic cells and the percent of DNA in the comet tail (TDNA%), tail length(TL), tail moment(TM) olive tail moment(OTM) and the rate of comet cells (Comet%) were selected as analyzing parameters. Bone marrow hematopoietic cells from patients with aplastic anemia examined with comet assay before and after IST separately, and the results were compared with those from 18 controls.ResultComet parameters from 91 patients with aplastic anemia including TDNA, TL, TM, OTM and Comet% were 5.02±3.95, 11.25±7.19, 1.70±2.02, 1.50±1.38 and 16.84±13.65 separately, all of which were obviously superior to those from control group respectively (p<0.05). The results were remained notablely higher when separately compared the comet parameters of SAA and NSAA with those of control group (p<0.05) , and there were no differences of the examined comet parameters of 58 SAA from those of 33 NSAA patients (p>0.05) .The parameters TDNA, TL, TM, OTM and Comet% were 4.43±3.64, 10.37±7.49, 1.37±1.61, 1.29±1.37 and 20.17±21.17 respectively 3-months later after IST in 53 SAA patients and those were 3.71±3.32, 10.04±7.18,1.15±1.80, 1.12±1.29 and 18.47±19.02 respectively 6-months later after IST in 30 SAA patients , which had no difference from those of 58 patients before IST (p>0.05) .There was no statistical difference in comet parameters of 18 SAA patients before and after IST (p>0.05) .ConclusionsBone marrow hematopoietic cells of aplastic anemia had intrinsic nature of genetic instabilities which were not seemed to be increased by IST. It may be impossible of IST being crucial in aplastic anemia evolving into clonal hematologic disorders.
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