| Objective To study the therapeutic effects of radix astragali injection(RA) and safflor injection(SI) on Cysteinyl aspartate specific protease-3(Caspase-3),Cysteinyl aspartate specific protease-8(Caspase-8) and apoptosis in rats brains following local cerebral ischemia/ reperfusion.Methods Male adult SD rats were randomly divided into five groups:the sham-operated group, the model group,the SI group,the RA group and the RA+SI group.The model of middle cerebral artery occlusion(MCAO) was established by thread ligation method,and rats were injected intraperitoneally corresponding medicine in different groups at 12h,30min before operation and every other 12h after operation.In sham-operated group and the model group, equally dose saline was injected intraperitoneally.Neurological system symptoms were evaluated at 12h,24h,48h after reperfusion.Then the rats were decapitated at 12h,24h,and 48h after reperfusion,and rats brains were taken for histopathological,TUNEL and immunohistochemistry examinations.Positive reacted cells of apoptosis,Caspase-8 and Caspase-3 are counted under light microscope at different time points of ischemia/reperfusion.Results The score of neurological system damage symptoms and the examination of histopathological,TUNEL,immunohistochemistry show that the cerebral ischemic damage in the RA group,the SI group and the RA+SI group was significantly milder than that in the model group(p＜0.05).Positive immune reacted cell amounts of Caspase-8 and Caspase-3 are alleviate in RA+SI group as compared with the RA group and the SI group in 12h,24h,48h ischemia/ reperfusion(p＜0.05).Conclusions The cooperation protective effect of RA and SI injection therapy against cerebral ischemia reperfusion injury might be associated with alleviating the positive expressions of Caspase-8 and Caspase-3.Moreover,The cooperation therapeutic effect of RA and SI is superior than the unity therapeutic effect of RA or SI.
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