Study On Application Of Supercritical Fluid Chromatography In 5 Drugs

Objective:To investigate the application of supercritical fluid chromatography in 2 chemicals and 3 chinese herbal drugs,and to determinate the contents of effective components in these 5 drugs by SFC.Contents:1.To establish a SFC method to determinate the three components in Akafen powder.2.To establish a SFC method to determinate the four components in Qutong tablet.3.To establish a SFC method to determinate the magnolol and honokiol in Officinal Magnolia Bark.4.To establish a SFC method to determinate the rutaecarpine and evodiamine in Medicinal Evodia Immature Frui.5.To establish a SFC method to determinate the dehydrocostuslactone and costunolide in Costusroot.Method:By taking the extraction rates of the main effective components in the 3 chinese herbal drugs as the evaluation index,single factor experiments were adopted to observe the influences on ultrasound extraction and microwave extraction, temperature,extracting time and methanol volume were selected as the examing factors.To study the effects of flow rate,modifier concentration,back pressure and column oven temperature on chromatographic resolution,to determinate the contents of effective components in these 5 drugs by optimal SFC method.Result:The optimal SFC parameters were using the Kromasil Slica column(250×4.6 mm i.d.,5μm) and setting back pressure of 200 bar,column oven temperature of 50℃,Modifier concentration of 18%and flow rate of 2.0 mL·min-1 for Akafen powder.Using the Kromasil Slica column(250×4.6 mm i.d.,5μm) and setting back pressure of 150 bar,column oven temperature of 60℃,Modifier concentration of 21 %and flow rate of 1.0 mL·min-1 for Qutong tablet.Using the Kromasil Slica column (250×4.6 mm i.d.,5μm) and setting back pressure of 200 bar,column oven temperature of 60℃,Modifier concentration of 5%and flow rate of 1.0 mL·min-1 for Officinal Magnolia Bark.Using the Kromasil Slica column(250×4.6 mm i.d.,5μm) and setting back pressure of 200 bar,column oven temperature of 60℃, Modifier concentration of 13%and flow rate of 2.0 mL·min-1 for Medicinal Evodia Immature Frui.Using the LiChrospher 100 Amino column(250×4.6 mm i.d.,5μm) and setting back pressure of 130 bar,column oven temperature of 60℃,Modifier concentration of 4%and flow rate of 1.0 mL·min-1 for Costusroot.Conclusion:The packed column SFC method have the advantage of quick analysis time,simple preparation of sample,low-cost organic solvent consumption and environment-friendly,etc.Besides,using supercritical carbon dioxide as mobile phase modified with little organic solvent help extend the useful life of packed column.In a word,The introduction of SFC to the area of traditional Chinese medicine is very promising.