Separation And Purification Of Phosphatidylcholine In Suaeda Liaotungensis Kitag And Its Antitumor Activity In Vitro

Seepweed is a high-quality vegetables, economic crops and plant resources with the value of health care. There are broad prospects for its development, but at present the development and utilization of seepweed are not enough.This study is on separation, purification and function analysis of phosphatidylcholine(PC)from Suaeda liaotungensis Kitag.The phosphatidylcholine, which purity is higher than 93% and the yield higher than 86%, was obtained by using aluminium oxide column chromatography, and optimizing the mobile phase as 95% ethanol: ammonia (100:1, V/V) mixed eluate. Compared with the method of single-eluting solvent, this method increased the selectivity of fixed phase to phospholipid, moreover there are no toxic residue in the final products.PC from seepweed has more advantages than from other organisms: low price and abundant sources of raw materials, no pesticide residues in products, no hormone residues, no antibiotic residues and other safety problems, less pollution of raw materials, no hidden dangers of transgenic food. It can meet the need for high-purity PC in medicine.Using the purified phosphatidylcholine on 7721, HepG2, MCF-7 and Hela cells culture,it showed a strong tumor suppressor activity, and a time, dose-dependent manner was found. The inhibition had a certain selectivity. The inhibition rate on 7721 and HepG2 cells were slightly higher than the other two cells. The PC from seepweed and Sigma standard product had the same role on inhibiting cancer cells'growing..