The Expression And Clinical Significance Of High Mobility Group Protein B1 In Peripheral Blood In Kawasaki Disease

Objective:Through detecting plasma level of HMGB1 in acute and convalescent phase of Kawasaki Disease, to explore the mechanism of high degree of activation of the immune system and Systemic inflammatory vascular skin changes and coronary artery lesion complication in Kawasaki Disease , Combined with a series of clinical symptoms to observe relationship between HMGB1and Kawasaki Disease , provide laboratory evidence and clinical guidance for the Symptomatic treatment and Reducing the complications such as coronary artery injury inKawasaki disease.Information and Method:Kawasaki disease group: To collect forty cases of Kawasaki Disease in heart medical department of Shanxi Province Children's Hospital, including twenty-seven boys and thirteen girls. According to echocardiogram, this group was divided into coronary artery lesion group(sixteen cases) and non-coronary artery lesion group(twenty-four cases).According course of development, this was divided into acute and convalescent phase. Normal control group:twenty cases of normal children who went to the hospital for health examination, including twelve boys and eight girls. To draw 2ml venous blood of normal chirdren and Kawasaki disease group in acute and convalescent phase. Saved plasma in -70℃ultra cold freezer after centrifuge, 37℃defrosted when specimen was enough.HMGB1 was measured by enzyme linked immunosorbent assay.Result:Three groups to compare show that:Compared with normal control group and convalescent phase of kawasaki disease,HMGB1 level was increased in the acute phase of Kawasaki disease.There was statistical significance in three groups (P<0.001). Both to compare show that: HMGB1 level in acute phase was higher than normal control group and convalescent phase; both had statistical significance (p<0.05). There was statistical significance between convalescent phase and normal control group(p<0.05). Compared with non-coronary artery lesion group in acute phase, coronary artery lesion group had statistical significance inHMGB1 Correlation analysis show that: HMGB1 had positive relation with coronary artery dilatation in Kawasaki disease r=0.921 P<0.05.There was statistical significance between coronary artery lesion and non-coronary artery lesion in convalescent phase. Correlation analysis show that: HMGB1 had positive relation with coronary artery dilatation in Kawasaki disease, r=0.906, P<0.05.Conclusion:HMGB1 level was increased significantly in acute phase of Kawasaki Disease; they reflected high degree of activation of the immune system and Systemic inflammatory vascular skin changes and coronary artery lesion complication in Kawasaki Disease.. these status has improved in convalescence phase,but patients still had Low immune function and vascular endothelial damage The change could have some significance In guiding the clinical symptomatic treatment .The change of HMGB1 had a relationship with coronary artery lesion.