Effects Of Eicosapentaenoic Acid On Proliferation Of HepG2 Cells

Objective To study the effect of eicosapentaenoic acid(EPA) on the proliferation of HepG2 cells and to explore its mechanisms.Methods HepG2 cells was in vitro cultured in different concentrations(0,15,30,45,60,75μl/ml) of EPA,The proliferation of HepG2 cells was detected by MTT method,and morphous was evaluated by fluorescent staining and transmission electron microscope,and apoptosis was detected by flow cytometry(FCM).Results Compared with control group,EPA can inhibit the proliferation of HepG2 cells in time-and concentration-dependent way.With the concentration increasing and time extension,the survival cells were taper.the inhibition ratio of the HepG2 cells in concentrations of EPA 45μg/ml and 60μg/ml is 36.7%and 52.1%,68.1%and 94.8%,94.2%and 99.7%on 24h,48h and 72h time period.An increasing of apoptosis was found by fluorescent staining,and obviously increasing of apoptosis body was found by TEM on the test group.the apoptosis of HepG2 cells induced by EPA was conformed by FCM.Conclusions The proliferation of HepG2 cells were inhibited by EPA and its effect depended on time and concentration of EPA.(2)The apoptosis of HepG2 cells can be detected by morphology and FCM and one of the mechanism may be related with apoptosis.