Study On The Cytotoxic Effect And Mechanism Of Ampelopsin On Human Bladder Cancer J82Cell Line In Vitro

Objective:The study aimed at researching the the cytotoxicity and mechanism of ampelopsin (AMP) on human bladder cancer J82cell line in vitro.Methods:1. The human bladder cancer J82cells were subcultured in vitro. The inhibitory effects on proliferation of human bladder cancer J82cell line were examined by MTT colorimetric assay.2. Cell apoptotic rate was detected by FCM using Annexin V/PI detection kit.3. The cell morphological changes and apoptotic bodies were observed by Transmission Electron Microscope (TEM).4. The expression of p-bcl-2and p-bad were detected by Western-Blot.Result:1. Compared with negative control group, the MTT colorimetric assay showed that AMP decreased the survival rate of J82cells in a concentration-dependent manner ranging from40μg/ml to70μg/ml in48h and72h. The IC50were57.16±1.86μg/ml and45.00±3.54μg/ml.2. The Annexin V-PI double staining show that the AMP grades concentration were able to induce the J82cell line apoptosis significantly.3. After ampelopsin40μg/ml and DDP2μg/ml used single dosing on human bladder cancer J82cell line after48h, we could observe apoptotic cells appeared under the transmission electron microscope.4.The AMP could down-regulate the expression of p-Bcl-2and p-Bad.Conclusion:1. AMP could inhibit the growth of people J82bladder cancer cell lines efficiently. And the inhibition has obvious concentration and time dependence.2. AMP could induce bladder cancer J82cells apoptosis significantly. The inducing mechanism may be related toown-regulate the expression of apoptosis protein p-bcl-2and p-bad.