| Objective:To study the proliferation and identification of dendritic cells (DC) derived from peripheral blood of Multiple scelerosis (MS) patients, monouclear cells, and investigate the chang of the epitope of thoes patients ,DC modificated by IFN-β1a.Methods:Mononuclear cells were isolated from peripheral blood of MS patients by Ficoll-Hypaque density and cultured in RPMI-1640 containing rhGM-CSF (100ng/mL),rhlI-4 (50ng/mL).After 9-day culture,the growth and morphology of DC were observed through the phase contrast or electron microscope,and their phenotypes were determined by flow cytometry.Results: The DC cultured in vitro turned into suspensive growth from adhesive situation on the 5th day ,then the number of DC increased continuously and the cells showed the irregular morphologic appearance of DC with veiled edges on the 9th day.Flow cytometry showed that the percentages of CD80,CD86,CD83 and HLA-DR on the blood-drived DCs from health control were (73.2±11)%,(90.4±5.2) %,(81.4±10.3) % and (91.3±5.9) % respectively. The frequency of CD86+ DCs and CD83+DCs were lower in MS patients compared to health controls(p<0.05);there were no differences for expression of CD80+ and HLA-DR+ DCs between the two groups (p>0.05). Aftter exposured to IFN-β1a in vitro, DCs from MS patients augmented percentages of CD86 and CD83 (p<0.05),but reduced HLA-DR, CD80 expression (p<0.05).Conclusions: A large quantities of DCs can be generated from mononuclear cells with rhGM-CSF (100ng/mL) and rhIL-4 (50ng/mL) ,wich is valuable to clinical application. One of the immunomodulatory effects of IFN-β1a treatment in MS may be a regulation of the autoimmune response through modifying the costimulatory molecules and antigen presention of DCs.
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