Biocompatibility Of Chitosan/nano-Hydroxyapatite Composite Scaffold Materials

Objective: To evalute the biocompatibility of the CS/nHA composite, and discuss the feasibility of it as a scaffold for tissue engineering. Method: The cytotoxicity of chitosan/nano-hydroxyapatite (CS/nHA) composite scaffold material was evaluated through the determination of the chondrocytes cells relative proliferation. The chondrocytes of rats were isolated and multiplied in vitro, the typeâ…¡collagen identified by immunocytochemistry , the chondrocytes stain with Saffranin O and toluidine blue. Then the chondrocytes were seeded onto the scaffold. The effect of the CS/nHA composite scaffold on the cell adhesion, proliferation, morphological changes were observed by the phase-contrast microscopy and scanning electron microscopy (SEM). The in vivo tissue response to CS/nHA was investigated by implanting into the subcutaneous and intramuscular sites. Result: The cytotoxicity of CS/nHA composite scaffold had been graded not larger than grade 1. And CS/nHA composite scafflod supported chondrocytes adhension and proliferation well. In vivo, The CS/nHA composite scaffold demonstrated favorable tissue compatibility without tissue necrosis and fibrosis. Conclusion: The CS/HA composite scaffold has good biocompatibilityand it will be a suitable scaffold for the osteochondral tissue engineering.