The Research About The Efficacy Adopting L6 Transposition To Reconstruct The Function Of Sciatic Nerve

Pelvic fracture is a common fracture in clinic, which may induce the Ventral rootavulsion.or ventral injury with the functional impairment of movement and feeling of lowerlimb. Nowadays, we have no efficient method to deal with them. To further study the injuryof sacral plexus root, we need animal experiments. Rat is the ideal model in the study ofsacral plexus root. in this article ,we emphasis on the animal researches of adopting thenormal sacral plexus root to Reconstruct the injured sacral plexus root. We use many ways toevaluate the the efficiency of this kind of therapy post-operation.PartOneObjective: To choose the ideal motor-nerve in rat.Methods: 20 adult SD rats were dissected to discuss the rat sciatic nerve anatomy.17rats's nerve roots of sciatic nerve are L4-6,While others are L4-5.and each tile has its twohypotypes. We also comfirmed that L4-5 is the permanent dominant nerve of biceps femoris,triceps surae and tibial muscle, and L6 is the main nerve dominant of triceps surae, but inthree rats, L6 has nothing to do with biceps femoris, triceps surae and tibial muscle.Results: our results indicate that the components of the sciatic nerve vary from L4 toL6.we also confirm the biceps femoris, triceps surae and tibial muscle's dominant nerve rootand contribution through EMG. So it builds a solid base of the choice of ideal motor-nerve .PartTwoObjective: To evaluate the efficiency of adopting L6 transposition to Reconstruct thesciatic nerveMethods: 30 adult SD rats were chosen to establish the animal model of sacral plexusroot avulsion at random after avulsing the right L4-6 out of intervertebral foramina. rats weredivided into 3 groups(n=10). In one group rats we did not reconstruct the sciatic nerve. In twogroups we adopted the L6 transposition to Reconstruct the injured sacral plexus root. After themodel establishment, the autologous sacral plexus root nerve was bridged with the right L6 nerve root by the translocation of the left L6 in one group , while the right L5 nerve root nervewas bridged by the translocation of the left L6 in other group. 12 weeks after operation, rat ineach group were selected for the histomorphology of the nerves was observed under themicroscope and the electron microscope. the models were evaluated by the observation of thesurvival rates of the rates, BBB scores, electron microscope weight and muscle fiberCSA(cross section area) of double biceps femoris, triceps surae and tibial muscle.Results: 12 week after operation, the BBB scores indicated significant differences(P=0.001)between 3 groups. We found remarkable significances between the ratio of weight and musclefiber CSA of double biceps femoris, triceps surae and tibial muscle. the Efficiency wasdifferent between the two groups (L6-L5 and L6-L6), the former was better. In the L6-L6 group,the recovery of biceps femoris, triceps surae and tibial muscle was different. The former wasbetter. The histomorphology of the nerves was observed under the microscope and the electromicroscope.Conclusion: L6 transposition combined with autograft of nerve root or without theautograft can reconstruct the partial function of sciatic nerve the in paraplegia rats. The effectof non-graft is better than that of graft obviously.