The Expression And Significance Of Estrogen Receptor Subtypes In Eutopic Endometrial Of Endometriosis And The Mouse Model

ObjectiveDiscussion of different estrogen receptor subtypes(ERαand ERβ) in eutopic endometrial of endometriosis(EMs)and normal endometrium(NEMs),as well as components to build this mouse model to quantify the gradient ectopic expression of lesions,studying different mechanism of estrogen receptor subtypes in the endometriosis pathogenesis.MethodsCollecting endometrial tissue of EMs Group 20 people and CINⅢGroup 20 people(both patients diagnosed as pathological late secretive phase),as well as organization of ectopic foci of the gradient quantifiable mouse model(0.2g Group,0.4g Group,0.6g Group) builded by two endometrial tissue groups of patients,detecting protein expression of ERαand ERβby Immunohistochemical staining methods (immunohistochemistry,IHC) to explore the relations of two receptors and the incidence of EMs.Results1,EMs Group and NEMs Group plant rates,respectively:0.2g Group:60%, 15%,P<0.05;0.4g Group:100%,85%,P>0.05;0.6g Group:100%,100%, P> 0.05.Lesion volume,respectively:0.2g Group:2.25±0.69 mm~3,0.38±0.21 mm~3; 0.4g Group:11.43±2.14mm~3,5.83±0.69mm~3;0.6g Group:21.56±3.40mm~3,12.01±2.46mm~3,in each group were P<0.05;plant amount and rate in two different endometrial:0.2gVs0.4g:P<0.05,0.4gVs 0.6g:P> 0.05;comparing both lesion volume:P<0.05.2,Expression of ERαin the EMs endometrial is 86.7%,which is significantly higher than the corresponding expression in ectopic foci(0.2g Group:46.7%;0.4g Group:56.7%;0.6g Group:56.7%),P<0.05.Expression of ERαin the EMs endometrial is 86.7%,which is significantly higher than the expression in the NEMs eutopic endometrial(56.7%),P <0.05.Expression of ERa in NEMs eutopic endometrial is not significantly higher than the corresponding ectopic foci(0.2g Group:43.3%;0.4g Group:43.3%;0.6g group:50%),P>0.05.3,Expression of ERβin the EMs endometrial is 66.7%,which is significantly lower than the corresponding expression in ectopic foci(0.2g Group:90%;0.4g Group:86.7%;0.6g Group:86.7%),P<0.05.Expression of ERβin the EMs endometrial expression is 66.7%,which is significantly higher than the expression in NEMs eutopic endometrial(30%),P<0.05.Expression of ERβin NEMs ectopic lesions (0.2g Group:50%;0.4g Group:63.3%;0.6g Group:60%) is not significantly higher than normal endometrial(30%),P> 0.05.Conclusion1,Compared eutopic endometrial in EMs Group with that in NEMS group,ectopic plant capacity of eutopic endometrial is higher,moreover the plant capacity increases with the amount to support the eutopic endometrial theory.2,In EMs,the main expression of eutopic endometrial is ERa and ERβ,the main expression of ectopic peritoneal foci is ERp,ERa expression is limited.ERβexpression has probably played a major role in happen and development of ectopic lesions.3,Blocking interaction between estrogen and estrogen receptor,especially blocking ERβmay provide a good treatment prospects for EMs.