| Passes: through observing Tong-Luo-Yi-Shen-soup to excise the excision of the kidney in the abdominal cavity and inject STZ to the the model big mouse with diabetes nephrosis (DN). Ordinary, to check up the blod sugar without eating meal(FBG), urine protein quota in 24 hour, the kidney function (blood serum urea nitrogen, myo- bitter wine), the triglyceride, the kidney organization TGF-β1and the kidney pathological change, confirmed Tong-Luo-Yi-Shen-soup to the DN model big mouse's treatment result and to protecting the function of the kidney, and discusses its action mechanism from the anti-kidney fibrosis angle.Method: After 60 SD big mouse repurchase, to feed them compatibly for a week, measures the weight, after examining the glucose in urine and the urine protein qualitative examination are negative, draw out 9 at random for normal control group good sham-operation (A group). To make the mold with the left kidney excision abdominal cavity to inject STZ to the other 51. And to make 43 big mice which the mold will succeed to divide into pathology control group 15 stochastically (B group), to eating Tong-Luo-Yi-Shen-soup treatment group 14 (C group), western medicine treatment control group 14 (D group).A, B group separately fill the stomach 10 weeks with the distilled water, C group for Tong-Luo-Yi-Shen-soup to fill the stomach, D group the suitable even suspension fills the stomach 10 weeks for Luo Ding Xin soup. After distinguishes before making the model ,after making model72h, for the medicine at the end of 4, 8,10W, fasting 12h, the tail vein draws blood measures empty stomach blood sugar (FBG).After the last medicine for 12h, the heart draws blood, heparin antifreeze, examination blood serum BUN, CREA;Selects the right kidney ,under the light microscope observes the kidney pathologicalchange; Uses the immunity group reduction, determinesthe kidney organization TGF-β1expression.Finally: After eating Tong-Luo-Yi-Shen- soup to treat for 10 weeks, to compares with the pathology control group, the Chinese native medicine treatment group, the western medicine treatment control group big mouse weight obviously increases (P<0.01), the empty stomach blood sugar obvio usly reduces (P<0.05), urine protein quota, the myo- bitter wine, the urea nitrogen, the kidney large index in 24h, TGF-β1expression obviously reduces (P<0.01);Compares with the western medicine treatment control group, the Chinese native medicine treatment group big mouse body weight increases obviously (P<0.05), the empty stomach blood sugar, the 24h urine protein quota, the blood serum myo- bitter wine is higher than the western medicine treatment control group, the urea nitrogen, the kidney large index, TGF-β1 is lower than the western medicine treatment control group, but does not have the significance difference (P<0.05), the kidney organization pathology damage reduces obviously.Conclusion: Tong-Luo-Yi-Shen-soup has certain treatment result to the DN model big mouse, and has certain protective function to the DN model big mouse kidney. Its action mechanism, possibly is through reduces the blood sugar, reduces the urine protein excretion, the control TGF-βexpression adjusts tubulus renalis epithelial cell transdifferentiation (EMT), the suppression interrenal nature fibrosis, thus displays to the DN big mouse kidney protective function.
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