Study On The Changes Of Nuclear Matrix Proteins During The Apoptosis Of Esophageal Cancer EC9706 Cells By Curcumin

In this study,the curcumin(Cur) was used to induce the human esophageal cancer EC9706 cells into apoptosis,and its effects were investigated by techniques of selective extraction-whole mount optic and transmission electron microscopy and proteomics.The differentially expressed nuclear matrix proteins were analyzed in order to explore the molecular mechanisms of a whole level.The results showed that the EC9706 cells were induced into apoptosis after being treated with 8μg/mL curcumin and the proliferation of EC9706 cells were inhibited, and the inhibitory rate was 69.9%.The results of flow cytometry analysis showed that the apoptotic peak appeared in EC9706 cells after treatment with Cur,the cell cycle was arrested in G₂/M phase.Light microscope and electron microscope showed that the malignant morphological and ultrastructural characteristics of EC9706 cells were changed after treatment with Cur,the morphology of the cells shrinked,cell nucleus concerntrated,chromatin agglutinated,mitochondria swelled,and apoptotic body formed.Immunocytochemistry result revealed that the expression level of wt-P53,Bax,Fas were significatntly upregulated while the expression level of Bcl-2 proteins was significantly downregulated in the cells treated with Cur.At the same time,the configuration of nuclear matrix-intermediate filament(NM-IF) was altered. 31 defferentially expressed nuclear matrix proteins appeared,12 of which was identified by MALDE-TOF-MS analysis.In conclusion,the configuration and composition of nuclear matrix were changed following apoptosis of the EC9706 cells,and it was possible to elucidate the molecular mechanism of apoptosis of EC9706 by studying the change of nuclear matrix.