Effects Of Vitamin C On The Expression Of Hyaluronic Acid Synthetase-2 Gene Matrix Metalloproteinase-1 Gene In Human Dermal Fibroblasts In Vitro

Objective:To explore the mechanism of vitamin C on delaying skin aging by observing the expression of hyaluronic acid synthetase-2 gene,matrix metalloproteinase-1 gene in human dermal fibroblasts in vitro.Methods:Human skin fibroblast were cultured in vitro.Then,these fibroblast cells were divided into 3 groups.The first group no vitamin C was added in the medium. The second one lower dimensional vitamin C was added in the medium.higher dimensional vitamin C was added in the medium in the third group.A few days later,we collected the fibroblast,which was index number growth phase from different groups,extracted total RNA,then amplifide by RT-PCR.The PCR product was determined by agarose gel electrophoresis,to analysis the mRNA of HAS-2 and MMP-1Results:Human skin fibroblast proliferates well in vitro,taking on typical Fusiform or babylon weeping willow leaf shap.Growth curve showed latency phase was shorter,,about 0-2 days,then index number growth phase.After about 5 days fibroblast cells proliferates stable,agarose gel electrophoresis showed,there was significant differences in the level of HAS-2 mRNA between any two groups except between the low-dose group and the control group,there was significant differences in the level of MMP-1 mRNA between any two groups except the high-dose group and the low-dose group.Conclusion:vitamin C can increase the transcription of HAS-2 gene,maybe increase synthesis of hyaluronic acid.it reduce the transcription of MMP-1 gene,the latter to reduce the degradation of collagen,increase in the thickness and elasticity of dermal,and reverse or delay aging of skin.