The Effects Of 17-β Estradiol On Hyaluronic Acid (HA) And Collagen Synthesis In Cultured Human Skin Fibroblasts

Objective Many reports have showed that skin aging after menopause in female may have relationship with the decrease of hyaluronic acid (HA) and collagen level. This report aims to explore the effects of 17-βestradiol on hyaluronic acid and collagen synthesis in cultured human skin fibroblasts.Methods The human skin fibroblasts were cultured in DMEM medium without phenol red in vitro. Serial dosages of 17-βestradiol were added to the culture system. Then the fibroblasts were divided into five groups according to the dosage of 17-βestradiol. They were control group, 1×10-11, 1×10-10, 1×10-9, 1×10-8mol/L 17-βestradiol group with 6 units in each group. After 48 hours the hyaluronic acid concentration in cell culture fluid was determined by ELISA method. The hydroxyproline concentration was determined by alkaline hydrolysis method to speculate the collagen synthesis ability.Results In the control group,10-11mol/L and 10-10mol/L 17-βestradiol groups the HA concentrations had no statistical significance (p>0.05). The HA concentration of 10-9 mol/L 17-βestradiol groups was higher than that of control group which had notable statistical significance (p<0.01). The HA concentration of 10-8 mol/L 17-βestradiol group was higher than that of control group which had statistical significance (p<0.05). But the difference of HA concentrations between 10-9 mol/L and 10-8 mol/L 17-βestradiol groups had no statistical significance (p>0.05). The difference of hydroxyproline concentration between 1×10-11 and 1×10-10 mol/L groups had statistical significance (p<0.05), in control group and 1×10-11mol/L, 1×10-10mol/L, 1×10-9mol/L 17-βestradiol groups the hydroxyproline concentration of the later group was higher than that of the former and the differences all had notable statistical significance (P<0.01). The hydroxyproline concentration of the 1×10-8 mol/L group is lower than that of the 1×10-9 mol/L group. The concentrations of HA and collagen showed positive correlation remarkably (r=0.794, p<0.01).Conclusions 17-Bestradiol could promote HA synthesis when its concentration is higher than some level. But the promotional effect may doesn't depend on the concentration of 17-Bestradiol. Hydroxyproline is highly special amino acid with constant content in collagen, then in general the hydroxyproline concentration could reflect collagen concentration. So the promotional effect of 17-Bestradiol on collagen synthesis in cultured human skin fibroblasts depends on its concentration when its concentration below 10-9 mol/L. But when its concentration is higher than 10-9 mol/1 the promotional effect may decrease. HA may influence the production of collagen. The influence of the change of estrogen level to the HA and collagen production in fibroblasts may be an important factor to accelerate skin aging in postmenopausal women. Estrogen may be a beneficial factor to delay skin aging in female.