An Experimental Study Of Effects Of Argon-helium Cryotherapy On Angiogenesis In Prostate Cancer

ObjectiveThe purpose of this study was to explore the effect of Argon-Helium Cryosurgery(AHCS) on the mice of bearing RM-1 prostate cancer through detecting angiogenesis and cell proliferation in prostate cancer of mice,To analyze the effects of cryotherapy on VEGF,Ki67 protein expression and microvesel density of prostate cancer,and provide an experimental evidence on cryoablation comprehensive treatment for advanced prostate cancer.Materials and MethodsA hundred mice experimental models with RM-1 prostate cancer were established,experimental mice were randomized into two group:the AHCS therapy group(group A,n=50),the tumor-bearing control group(group B,n=50).after the AHCS therapy,Peripheral tumor tissues of 50 mice were obtained from group A at 1d,3d,5d,7d,14d.at the same time,the tumor-bearing control group B(not do the AHCS therapy),tumor tissues of 50 mice were obtained from group B at 1d,3d,5d, 7d.14d.MVD,VEGF,Ki67 protein expression were detected by immunohistochemical staining at different time after the freezing.Results1.Prostate cancer cell has stable biology characteristics,and the incadence of cancer was 100%.2.MVD,VEGF and Ki67 protein expressions in freezing group were decreased.The lowest value was detected at the third day after freezing.The value increased slowly after that.There was no change in MVD and VEGF,Ki67 protein expressions at different time in control group(P＞0.05),There were significant change in MVD,VEGF,Ki67 protein expressions at different time in freezing group(P＜0.05). Correlation analysis showed suggest a positive correlation between VEGF protein expression and MVD(r=0.8793,P＜0.01) suggest a positive correlation between ki67 protein expression and MVD(r=0.7614,P＜0.01).suggest a positive correlation between VEGF protein expression and ki67(r=0.6921,P＜0.01).Conclusion After argon-helium cryoablative treatment for prostate cancer,local tumor MVD, VEGF,Ki67 was reduced at the first day,the lowest value was detected at the third day after freezing,then increase,in the dynamic process of change,MVD,VEGF, Ki67was positively correlated.And cryoablation joint anti-angiogenesis is an effective means that may be integrated in the treatment of advanced prostate cancer.