Study On Changes Of Blood Viscosity And NGB Expression And Their Dependablity By Brain Injury In Rats

AIMS:To observe the changes of blood viscosity and NGB expression in the brain-traumatic rats model established by free falling striking.METHODS:1. Weights-free-falling brain injury models were established by the method of free a weughts to cause brain injury. Mixed venous blood from right atrium were taken for detect blood viscosity variations by blood viscograph analysis meter.2. After establishing the brain-traumatic rats models, the expressions of NGB in cortex cells of the rats in different times after brain trauma were detected by chemical method. MOTAC pathological images system were used to analysis and measure the integral optical density values of the positive chemical labeled NGB.RESULTS:1. After being injured, the blood viscosity of the brain-traumatic rats was all higher than that of the control group (P<0.05). The injured-30min group and injured-Id group displayed two maximum changes of blood viscosity, and the injured-8h group and injured-5d group manifested two minimum changes of blood viscosity.2. The expression of NGB started to increase from 10min after being injured, and reached the first peak in 2h, and then dropped slightly. The expressions of NGB reached the peak again in 1d after being injured, then gradually dropped to the same level as controls from 5d to 7d.CONCLUSIONS:1. Brain trauma of rats can cause the increase of blood viscosity; the extent of increase is different along with the time of trauma. Two peaks emerged in the 30min and 1d after being injured.2. Primarily and secondary brain injury can all to result in increase of NGB expression. NGB may participate the brain injury stress,ischemia,hypoxia compensation, protecte Neurons, delay the damage proceeding.3. NGB expression changed significantly by the blood viscosity increased.