Study On The Mechanism Of Globus Hystericus Interfered By BanFo Nanometer Micro-Pill

Globus hystericus is the frequently-occurring disease of stomach intestine functional disease.It unconsciously presents as psychiatrical and neurological symptoms and globus sensation with patients'condition waving,lingering and difficult to cure.It has a bad influence on work and life of amplitude patients.At present,the study on its pathogenesis and ideal treatment is insufficient. Now Pinellia and Magnolia Bark Decoction has obtained a good effect on globus hystericus.And BanFo Nanometer Micro-Pill which is based on Pinellia and Magnolia Bark Decoction product with the new nanotechnology. For further study and trying to find out the mechanism of globus hystericus interfered by BanFo Nanometer Micro-Pill,the study whcih is subsidized by Jiangsu Scinence And Technology Department collect the clinical cases and built the animal model with chronic pharyngitis by using ammonia and turpentine to the pharyngeal part of the rabbit and the animal depression model by exposing to an unpredictanle sequence of stressors to the rat. Clinical research【Objective】investigated the distribution rule and the pathogenesis of the characteristics of traditional Chinese medical syndrome in Globus hystericus by the retrospective analysis of the patient【Methos】use the approch of cross-sectional study,adopt the on-the-spot investigation method,let respondents fill in the Globus hystericus questionnaire,combine the clinical examination results,gather the respondent's information material of the four diagnosis methods of the traditional Chinese medicine through doctor's interview at the same time and carry on differentiation of symptoms and signs according to the relevant syndroms standard of Chinese medicine.Then,collect the information of the qualified questionnaires and enter database,use SPSS10.0 software to study the relevant influence factor,the distribution of the Chinese medicine syndromes and the characteristic of Globus hystericus.【Results】the distribution of the Chinese medicine syndromes and the characteristic of Globus hystericus was that the Chinese medicine syndromes of Globus hystericus correlate to gan,pi;which respectively accounted to 83.1%,12.1%.【Conclusion】The inducements of Globus hystericus are relevant to the sentiment,season,diet and fatigue.its main places of disease is gan,pi.And the key of pathogenesis is liver depression. The animal model with chronic pharyngitis:【Objective】To explor the influence of Globus hystericus interfered by BanFo Nanometer Micro-Pill which chose the following figures as the blood rheology, the pharyngeal part pathologic morphology and some basic figures of the chronic pharyngitis.【Methos】To divide randomly rabbit into 6 groups, the normal control group (NS), the chronic pharyngitis model group,the positive group (Man Yan Shu Ning), BanFo Nanometer Micro-Pill in low, medium, high dosage, there were 8 rabbits in each group. the study built the animal model with chronic pharyngitis by using ammonia and turpentine to the pharyngeal part of the rabbit. Regularly observed the weight,rectal temperature and behavior of the rabbit,and inspected the blood of the rabbit.At the end of the study,observed the pharyngeal part pathologic morphology of the rabbit.【Results】BanFo Nanometer Micro-Pill can reduce the quantity of the chronic inflammatory cells (lymphocyte, monocyte) in the animal blood, alleviate permeability of the blood vessel walls of the pharyngeal department and exfiltration of inflammatory cells, and repress proliferation of the fibrous tissue,and can improve the blood rheology, strengthen the immune function.【Conclusion】the results of the research showed that the remedial functions of BanFo Nanometer Micro-Pill were realized by many paths, such as anti一inflammation, improvement permeability of pharyngeal department blood vessel, alleviate the inflammation exfiltration, inhibition fiber proliferation, improvement the blood rheology, strengthen immunity etc.The animal depression model:【Objective】To investigate the effect of BanFo Nanometer Micro-Pill on the behaviors of depressed animal and detect the amount of monoamine neurotransmitte in brain of rats. To approach preliminary the antidepressant mechanism of mechanism of BanFo Nanometer Micro-Pill.【Methods】To divide randomly adult Sprague-Dawley (SD) rats into 6 groups, the normal control group (NS), the depression model group, the positive group (Fluoxetine), BanFo Nanometer Micro-Pill in low, medium, high dosage, there were 12 rats in each group. All rats were administered medicine by intragastric administration. Model. Isolated-living condition and chronic mild unpredictable stress with being bred in a single cage are applied successively to set up the rat depression model except normal control group, that it is using seven different stressors (the reverse of day and night, the swimming in ice water, shaking, clamping the tail,the prohibition against water, abrosia, polluting the cage). Regularly observing the behavior of the rats by the open-field tests.At the end of the study,detecting the amount of monoamine neurotransmitte in brain of rats.【Results】BanFo Nanometer Micro-Pill can improve obviously behavioral index and show curative and preventative effect to the animal depression model,and it also can increase obviously the amount of monoamine neurotransmitter (NE, 5-HT, 5-HIAA) in the animal depression model, which may be one of the mechanisms of its anti-depressive action.