Experimental Study On The Effect Of Wuyou Decoction On Behavior And Monoamine Neurotransmitters Of Rats With Depression

Objective:Casual observation of Wuyou Decoction in chronic unpredictable mild stress andsolitary keep depression model rats behavior and brain monoamine neurotransmitters5-hydroxytryptamine and norepinephrine effects of serotonin. Explore its mechanismand provide the theory for clinical medication.Methods:1, Model preparation: Only60SD clean level male rats, weight200±20g. Byweight groups within the group of rats KuWeiSuan to make points after mark,according to the group of the whole amount of group simple random sampling isdivided into six groups: the normal control group, the model group, the high does ofWuyou Decoction group, the middle and low dose of Wuyou Decoction group andVenlafaxine group, each group of10only. Normal control group every cage5onlyraise, will not be model preparation. The other5rats with a solitary and chronic mildcannot foresight stress sexual stimulation as a method for depression made ratsmodule, and stimulate21days. Made before the stare of mould, big rats adaptabilityraise1week.2, Feeding on medicine in different groups: Made after the success of the mould,Wuyou Decoction of high, middle and low dose group of11.4g/kg per day,5.7g/kg,2.85g/kg to fill the stomach medicine, normal control group and model group daily fill the stomach3ml physiological saline, Venlafaxine group to12.5mg/kg daily fill thestomach to medicine. In the experiment date22for medicine for3weeks, once a day.3, Index detection: Rats gaining weight change, food consumption, sugar waterconsumption and Open-field test as behavioral indicators. TO die before, respectivelymade the dug treatment of21days and method; evaluate Wuyou Decoction forchronic stress depression model of behavior change rats. Brain5-HT, NE contentdetermination of filling in for21days after the stomach medicine, rapid Bao Qu ratsorganization, after homogenate speed centrifugal, take on the liquid, the enzymeleague immune method(ELISA) is the test, and statistical analysis.Results:1, After21days chronic mild cannot foresight stress and solitary raise, comparedwith normal, each made module rats of behavioral indexes is reduced and thedifference was statistically significant(p<0.05or p<0.01), the results show that themodel success made.2, After21days after the dug intervention, and model group comparison, Chinesetraditional medicine Wuyou Decoction in high, middle dose group and westernVenlafaxine rats, weight increased significantly(p<0.05); food consumptionsignificantly increased(p<0.05); Activities increased significantly(p<0.05); The sugarwater partial a enhanced obviously(p<0.05); Brain single amine neurotransmitter5-HT, NE content increased significantly(p<0.05), a statistically significantdifference.Conclusions:1, A lone with chronic mild unforeseen stress sexual stimulation can be preparedby an ideal depression animal model. The make, model rats and lake of interest in thepleasant sensation, activity decrease, explore and depression reduced ability to samplebehavior, very good to simulate the clinical depression of depression in state.2, Wuyou Decoction can significantly improve the abnormal behavior depressionof experimental animals, increase brain monoamine neural transmitters5-HT, NE content, and a certain concentration-response relationship.3, Wuyou Decoction with antidepressants, its mechanism may increase with brainof monoamine transmitters in5-HT, NE relevant content.