| Background:The colon cancer is one of the digestive tract malignant tumors, and the all world approximately has 500,000 people to be taken bad every year.The mortality rate of the colon cancer at the Western country is only next to the lung and the breast cancer.And that ranks the third in malignant tumor mortality rate~[1].In recent years,the incidence and mortality trends of China's colorectal cancer was also increased.According to the 1993-1997 Chinese 1/10 the population sample survey results showed that the cause of death~[2]The death of colon cancer is the fifth cause of in the Chinese malignant tumor.In the clinical treatment of patients with colorectal cancer,of which about 1/3 patients when they seek medical treatment to the tumor has advanced stage that should not have to remove or diffuse metastasis,poor prognosis of these patients,the average survival time is about 7 months.While the other 2/3 of patients even if the original tumor can do it "radical resection",but still about 50%of patients survive a period of not more than five years,most of which (about 40%) patients died of liver metastasis.How has the early prediction that colorectal cancer metastasis,the medical specialist has always been an issue of great concern..Hepatocyte growth factor(HGF) is a multifunctional cytokine.Research has shown that,HGF can regulate cell growth and exercise,and promote generation about the various cell morphology,is the body fluids medium of interaction of epithelial cells and mesenchymal cells.HGF has strong functions to the mitogenesis,the tissues formation,inducing epithelium migration and invasion,and inducing vasculogenesis. The combination of HGF and the c-met in the tumour cells results in acceptor autophosphorylation,and enhanced the activity of Tyrosine Kinase.So it was bring about many kinds of substrate proteins the tyrosine phosphorylation.As a result of high expression and forming a positive feedback of HGF and c-met in the tumor tissues,the tumor became indefinite growth and Invasion.And this positive feedback action has been confirmed in the malignant tumor such as the gliomas,osteosarcoma, breast cancer and multiple myeloma,etc.And this positive feedback action has been confirmed in the malignant tumor such as the gliomas,osteosarcoma,breast cancer and multiple myeloma,etc.It indicated that HGF in body liquid of patient with malignant is significantly elevated.And this positive feedback action has been confirmed in the many malignant tumor such as the gliomas,osteosarcoma,breast cancer and multiple myeloma,etc.It indicated that HGF in body liquid of patient with malignant is significantly elevated and concerns invasion status very nearly.Although there is many scholars at home and abroad to studied HGF in various malignant,but mostly cases limited to research in tumor tissues.HGF have been consider to be bound up with liver cancer metastasis through the study of HGF and acceptor's expression in liver cancer tissues by Xie Qian,Luo Yun Quan,etc. Eichbaum MH,etc reported that HGF consistence in the breast cancer tissues is an independent prognostic indicators.In the last years,there are more and more researches with reference to contents of HGF in the serum.Tanaka etc.researches indicated that HGF in the serum of the early and advanced gastric cancer is significantly higher than the normal control group.It prompted that the serum HGF levels may have clinical value as a tumor marker in the early gastric cancer and advanced gastric cancer.YIN Jia-jun,etc.were also reported the serum HGF concentrations in breast cancer metastasis group were significantly higher than those without recurrence and metastasis group and control group.Although there is foreign specialist to have been study about the relationship with serum HGF levels and stages of colorectal cancer,but the researches in the liver metastasis of colorectal cancer is still confined to the tissues,especially investigation in the serum expression is still rarely.Purpose:In view of the colorectal cancer whether have been liver metastases, most of them are to rely on imaging such as BUS,CT or MR,and postoperatively or after treatment,such as serum CEA to determine whether the increase.The one's illness often has taken a turn for the last stage when the imaging has been diagnosis. Not only clinical treatment has been brought about difficulties,and the patient's prognosis has been poor.Therefore,the current clinical practice is usually taken interventional chemotherapy after the postoperative of colorectal cancer to prevent the possibility of liver metastasis.How can the early prediction for colorectal cancer whether have been transformed into liver,there are great significance for improvement prognosis prediction and guidance of more effective treatment methods. In this study,there are 52 patients with colorectal cancer as the research object.We use the ELISA to mensurate the HGF in serum on those patients,and then analyze the relationship between HGF and colorectal cancer patients with liver metastasis. Attempt to explore the value of HGF on colorectal cancer patients with liver metastasis.Mehod:Since June 2004 to December 2006,we selected 52 cases of patients with colorectal cancer.All of patients have been confirmed by pathology.Among them,the male:female was 27:25,ages range from 31 to 75,the average age is 54.17 years old.According to Duke's stages,52 patients divide into stage A and B 13 cases, stage C 17 cases,stage D 22 cases.Among the stage D,17 cases have the liver metastasis(all have imaging diagnosis),5 cases have other parts of the transfer (Non-liver metastasis).Another 20 cases serve as healthy controls.Experimental methods are conducted in accordance with Human HGF detection kit (RD America Inc.products,labeling:063,402) instructions which purchased from Guangzhou Kang companies.We use SPSS 13.0 software to deal with all data.Using independent samples t test and One-Way ANOVA test,when P＜0.05 considered statistically significant.Results:1.The serum HGF values comparisons between the experimental group and control groupAll research object with colorectal cancer,when they were selected,had have been diagnosed and had been confirmed by pathology.At the same time,the control group is composed of 20 healthy cases.The results will input SPSS13.0 software package and will calculate and analysis.Through the inspection found that the HGF content in the serum of patients with colorectal cancer is commonly higher,and there are significant differences(P＜0.005) between the experimental group and the healthy control group.It shows that serum HGF levels of patients with colorectal cancer are much higher than that of the healthy population.2.Comparison serum HGF contents between the two groups whether the liver metastasis.All subjects divided into two groups after various test such as BUS,CT etc,one group is liver metastasis and another one is no liver metastasis.Through the statistical analysis,the serum HGF contents of liver metastasis group were much higher than that of no metastasis group(P＜0.005).In addition to the patients with liver metastasis,we also found that the serum HGF contents of the patients with other places metastasis were also much higher than that of patients of no liver metastasis.It was show that the HGF released not only when the liver itself suffering from damage, but also when the tumor cell detachment from primary malignant tumor itself. 3.Relationship with each different staging of colon cancers about HGF contents in serumJust as like grouping of liver metastasis or no,we will divide colorectal cancer patients intoⅣstages according to Duck stage.From results of experimental,we can also found that the serum HGF content have a very close relationship with colorectal cancer stages.And the latter the stage is,the higher the HGF content in serum will be (P＜0.005).It can be seen that the HGF content in serum of colorectal cancer patients not only can be used as a marker of colorectal cancer,but also can be used as an indicator to prediction of colorectal cancer whether the liver metastasis.Conclusion:Through this experimental study found that patients with colorectal cancer compared with the control groups,HGF values rising were significantly different.In addition,through this experimental study we found that HGF also has relationship with colorectal cancer stage.And the later the colorectal cancer's stage is, the higher the serum HGF value will be.Especially the serum HGF expression in patients who have liver metastases was far higher than those without liver metastasis group.Thus this result farther prompted the serum HGF in colorectal carcinoma may indicate a more aggressive biological behavior.However it is worth mentioning of only 5 patients in the Duck'D that had other parts of the transfer(no liver metastases). Although they had the higher value of serum HGF,but had not significant difference to compare with Duck'A and B.Analysis of its causes may be related to the small number of cases.In short,the serum HGF values are likely to become a kind of tumor markers to be used diagnosis for colorectal cancer patients,and may be have certain of guiding significance for colorectal cancer patients at metastasis,stages,prognosis judgement and develop treatment programs.
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