The Study Of Genetic Expression Of TPD52L2,PTHLH,DNAJC11,CD8B1,MARK3 On The Peripheral Blood Of Aggressive Periodontitis

It has been suggested that Aggressive periodontitis(AgP) is one of the main reasons that causes young people to lose their teeth,which has a genetic background and family aggregation.This disease is developed very rapidly.The classification of Aggressive periodontitis is ambiguity and the etiology and pathogenesis of Aggressive periodontitis are not clear until today.Not with standing,some microorganisms with special virulent factor could cause AgP.Currently,it is considered that the etiopathogenisis of AgP includes host susceptibility,environmental factor and pathogenic microorganism and the predisposing factor of host is decided by related genes.Evidences also indicate that Aggressive periodontitis may be involved with dysfunction of the cellular immune responses.Our previous researchers have found that two groups genes(up-regulation and down-regulation) which could level express differently in AgP group compared to normal group by using microarray technology,but there are false positive and false negative in the result of gene chip.In order to confirm the result of the gene chip as well as find the AgP related specificity gene.Here we selected 5 genes which associated with immune responses closely from 48 significant differentially expressed genes.They are TPD52L2,PTHLH,DNAJC11, CD8B1 and MARK3.We detected the RNA transcriptional expression level in AgP and normal control groups by using FQ-PCR to examine the result of gene chip,then promote the research development of late stage.Object:To detect the RNA transcriptional level of TPD52L2,PTHLH, DNAJC11,CD8B1 and MARK3 which genes are express differently on peripheral blood in AgP by FQ-PCR.These can authenticate the results of gene chip and facilitate the later research on the etiopathogenisis and pathogenetic of AgP. Methods:5ml blood of peripheral vein was been taken suction from every patient and healthy controls.We isolated the leucocytes from every sample.And reverse transcripted cDNA,used RT-PCR technique to detect the express level of the 5 genes.Result:Comparing with controls,the results of FQ-PCR of TPD52L2, DNAJC11 and PTHLH are higher,which are consistent with the gene chip.In the gene expression of the peripheral blood leukocyte,TPD52L2 is higer 9.71 times,PTHLH is higer 4.47 times,and DNAJCll is higer 6.36 times.But the CD8B1 in the FQ-PCR examination demonstrated it is higer 5.62 times,it is obvious difference in the down-regulation result of the gene chip.Then the result of MARK3 does not have the significant difference comparing with the gene chip.Conclusion:The differential expression of TPD52L2,DNAJC11 and PTHLH may be related to the pathogenesis of Aggressive periodontitis.The express high level in AgP suggest that this gene may be associated with the genetic predisposition to aggressive periodontitis,but its accurate relevance also waits for further studies.