The Expression And Significance Of PPARγ And MMP-9 In Pulmonary Vascular Endothelial Cells With Chronic Obstructive Pulmonary Disease

Objective:In order to investigate the effect of peroxisome proliferators activated receptor gamma and matrix metalloproteinase-9 in pulmonary vascular inflammation and remodeling in patients with chronic obstructive pulmonary disease(COPD) by detecting the expression of PPARγand MMP-9 of pulmonary vascular endothelial cells.Methods:Lung specimens were collected from the male patients who with lung carcinoma and smoking history and need to be operated in thoracic surgery,30 patients were divided into 2 groups, control group:patients with normal pulmonary function(non-COPD group) and patients with COPD (COPD group),each of 15 cases,matched with age.Air flow obstruction was determined by pulmonary function test and pulmonary artery pressure were examined by continuous wave Doppler echocardiographic measurement of the tricuspid regurgitant jet,and the smoking index were recorded in all patients before the operations.The remodeling of pulmonary artery remodeling was observed with HE staining under microscope and triple strains,lumen area,arteries walls,intimal area,muscular arteries,partial muscular arteries,non partial muscular arteries were measured,the expression of PPARγand MMP-9 of pulmonary vascular endothelial cells were measured by means of immunohisto-chemistry,analysis were made.Results:1) Normal pulmonary vascular endothelial cells express PPARγ,the expression of PPARγin control group(12.28%±4.23%) was higher than that in COPD group(2.65%±0.89%)(P <0.01 ),and the expression of MMP-9 in COPD group(38.53%±11.73%)was higher than that in control group(12.83%±3.89%).2) Pulmonary function in COPD group drops compared with control group.3) Characteristics of intra-acinar pulmonary muscular arteries:Compared with control group, arteries walls of COPD group congested and swelled,inflammation cells increased,the thickness of intimal layer,walls of intra-acinar pulmonary muscular arteries was significantly higher,the area of its lumen was narrow and the proportion of their muscular arteries was higher,the area of collagen fibrils was increased.4) Correlation analysis:An negative correlations were showed with the expression of PPARγand MMP-9(r = -0.85,p<0.01 ),and inflammation cells,the pulmonary artery of the artery muscle ratio,the area of collagen fibrils,negative correlation was showed(r=-0.604,-0.759,-0.485,-0.862,P< 0.01);An positive correlations were showed in the expression of MMP-9 and inflammation cells(r=0.524,P<0.01 ),an positive correlation was showed with the pulmonary artery of the artery muscle ratio,the area of collagen fibrils,(r= 0.961,0.574,0.954,P<0.01);the artery wall area and the area of collagen fibrils showed positive correlations(r = 0.878,p< 0.01).an positive correlation was showed with the smoking index and the ratio of muscular arteries,partial muscular arteries,the area of collagen flbrils(r=0.889,0.764,P<0.01).Conclusion:Patients with smoking and COPD have pulmonary vascular inflammation and remodeling,MMP-9 of pulmonary vascular endothelial cells was involved in the inflammation and remodeling process.,but PPARγwas involved in the anti-inflammation and anti-remodeling process.