The Effects Of Extract Of Ginkgo Biloba And Rosiglitazone On The Pancreatic NF-κB Expression And β Cell Apoptosis Of High-fat Diet Rats

Objective: To explore the expression of pancreatic islets Nuclear factor-κB, inflammatory mediator IL-1βand pancreatic isletsβcell apoptosis in insulin resistance rats model induced by high-fat diet, and to explore the effects of rosiglitazone and Extract of Ginkgo Biloba on improving insulin resistance andβcell apoptosis.Methods: 64 male Wistar rats were divided into normal control diet(NC)group(n=16)and high-fat diet(HF)group(n=48). After 4 weeks of feeding, hyperinsulinemia-euglycemic clamp technique test was preformed in 8 rats of both group.Then the rats in high-fat diet group were divided into high-fat control (HF)group, rosiglitazone (RSG) group and Extract of Ginkgo Biloba 1, 2, 3(EGB1, EGB2, EGB3)group(n=8).After 8 weeks of feeding, Oral glucose tolerance test and hyperinsulinemia-euglycemic clamp technique test were preformed once again. After the clamp, the rats were sacrificed by depletion,then the serum lipid, insulin, free fat acid (FFA) were examined, the IL-1βconcertration in blood was detected by ELISA,βcell apoptosis was evaluated by TUNEL, pancreatic NF-κB expression was measured by western blot.Results: Compared with NC group, glucose infusion rat(GIR) was lower in HF group after 4 weeks, Compared with NC group, pancreatic NF-κB expression in HF group was increased after 8 weeks, and treat of rosiglitazone prevented the increasing; the apoptotic rate ofβcell was higher in HF group, and using rosiglitazone and Extract of Ginkgo Biloba prevent it's heighten; the IL-1βconcertration was higher in HF group after 8 weeks, intervision of rosiglitazone and Extract of Ginkgo Biloba decreased IL-1βconcertration.Conclusion: Insulin resistance rat model can be induced by 4 weeks of high-fat diet. IL-1βconcerntration and NF-κB protein expression increased in insulin resistance rat and pancreaticβcell apoptosis was higher in high fat diet rat,indicated. Rosiglitazone and Extract of Ginkgo Biloba may improve inflammatory state and inhanceβcell function of insulin resistance rat by decreasing IL-1βand NF-κB expression.