The Role Of IL-8 And ERβ And Their Correlation In The Occurrence And Progression Of Ovarian Epithelial Tumors

Objective: Ovarian tumor is a common tumor in female reproductive system, and malignant ovarian tumor is one of the three malignant tumors in female reproductive system. The incidence of malignant ovarian tumors takes up 22.9% among the common female malignant tumors, and the incidence of ovarian epithelial carcinoma takes up 85-90% among all the malignant ovarian tumors. Although rapid development in terms of operation, chemotherapy and radiotherapy has been made in recent years, until now there is still no effective therapy for malignant ovarian tumors, resulting in the fact that the five-year survival rate among patients with it is only about 25-30%. With the development of diagnosis technology and the improvement of treatment in carcinoma of cervix and ovarian endometrioid carcinoma, ovarian epithelial carcinoma has become one of the most serious life-threatening tumors to women. Thus, doing research on the occurrence and progression of ovarian epithelial tumors is of great significance to its diagnosis and treatment.At present, there has been no report on the protein expression of IL-8 and ERβand their correlation in ovarian epithelial tumors, thus, the correlative role they play in the occurrence and progression of ovarian epithelial tumors is unclear. Through examining the protein expression of IL-8 and ERβin 72 cases of normal ovarian tissues and the tissues of ovarian epithelial tumors by the immunohistochemical method, this thesis primarily explored the role and correlation of the protein of IL-8 and ERβin the occurrence and progression of ovarian epithelial tumors, with the purpose of clarifying the applied value of IL-8 and ERβin the diagnosis and treatment of ovarian epithelial tumors and providing new approach and basis for its basic research and clinical diagnosis and treatment.Methods: 1 Subjects: Seventy-two samples were selected from the fresh normal ovarian tissues and the tissues of ovarian epithelial tumors in patients aged 37-79 (mean age 61) who had been admitted into The Fourth Hospital attached to Hebei Medical University, including 9 cases of normal ovarian tissues, 18 cases of benign ovarian tumor tissues and 45 cases of malignant ovarian tumor tissues (17 cases of ovarian serous cystadenocarcinoma, 16 cases of ovarian mucinous cystadeno- carcinoma and 12 cases of ovarian endometrioid carcinoma). Among the 45 cases, using the clinical stage standards of FIGO, there were 17 cases of stage I and II and 28 cases of III and IV; using the method of histological grade, there were 16 well-differentiated cases and 29 moderately and poorly- differentiated cases; 30 cases had ascites and metastasis occurred in 26 cases. 2 Method: All samples were taken within 30 minutes-1 hour after separation from the body, fixed in 10% Formalin, dehydrated routinely, made transparent, embedded in paraffin and then cut into serial sections, each with a 4μm thickness. These sections were diagnosed and identified by the experienced pathologist, and stained through the method of S-P immunohistochemistry. SPSS11.5 software was used to analyze the data and P<0.05 was considered to be statistically significant.Results: 1. The expression of IL-8 in normal ovarian tissues,the tissues of benign ovarian epithelial tumors and ovarian epithelial carcinoma: the protein expression of IL-8 was located in the cell cytoplasm and its positive expression was the brown granules in the cell cytoplasm. The positive expression rate of IL-8 in ovarian epithelial carcinoma (84.4%) was higher than that in benign ovarian epithelial tumors (55.6%) and that in normal ovarian tissues (22.2%), being statistically significant (P<0.05); while the expression of IL-8 in normal ovarian tissues and benign ovarian epithelial tumors was not significantly different (P>0.05). 2. Among the clinicopathological parameters of ovarian epithelial carcinoma (age, pathological type, histological grade, ascites, clinical stage, lymph node metastasis), the protein expression of IL-8 was correlated with the clinical stage. The positive expression rate (96.4%) of IL-8 in stage III and IV was higher than that (40.7%) in stage I and II, having significant difference (P<0.05). And the protein expression of IL-8 went up with the increase of clinical stage, which had nothing to do with age, pathological type, histological grade, ascites, lymph node metastasis, and so on. 3. The protein expression of ERβin normal ovarian tissues,benign ovarian epithelial tumors and ovarian epithelial carcinoma was mainly located in ovarian epithelial cells or cancer cell nucleus and occasionally located in interstitial nucleus, and the positive cell nucleus of ERβwas yellow or brown. The protein expression rate (37.8%) of ERβin ovarian epithelial carcinoma was lower than that (66.7%) in benign ovarian epithelial tumors and that (77.8%) in normal ovarian tissues, having significant difference (P<0.05); while the protein expression of ERβin normal ovarian tissues and that in benign ovarian epithelial tumors had no significant difference (P>0.05). 4. Among the clinicopathological parameters of ovarian epithelial carcinoma, the positive protein expression rate (11.8%) of ERβin ovarian serous carcinoma was obviously lower than that (56.3%) in ovarian mucinous carcinoma and that (50%) in ovarian endometrioid carcinoma, having significant difference (P<0.05), which had nothing to do with age, histological grade, ascites, clinical stage, lymph node metastasis, and so on. That is to say, the protein expression of ERβwas only related to histological subtype, being lower in ovarian serous carcinoma than in ovarian mucinous carcinoma and in ovarian endometrioid carcinoma. 5. The protein expression of IL-8 had negative correlation with the protein expression of ERβin normal ovarian tissues, benign ovarian epithelial tumors and ovarian epithelial carcinoma (r=-0.619, P<0.001). Conclusions: The protein expression of IL-8 in ovarian epithelial carcinoma was higher than that in benign ovarian epithelial tumors and that in normal ovarian tissues. The protein expression of IL-8 went up with the increase of clinical stage, indicating that IL-8 could induce the carcinogenesis and progression of ovarian epithelial tumors; The protein expression of ERβin ovarian epithelial carcinoma was lower than that in benign ovarian epithelial tumors and that in normal ovarian tissues, indicating that, from normal ovarian tissues to ovarian carcinoma, the decrease in ERβmight play a critical role and ERβfunctioned differently in ovarian epithelial carcinoma of different pathological types. The expression of IL-8 and that of ERβwere obviously negatively related, which indicated that they might play negatively correlative roles in the occurrence and progression of ovarian epithelial tumors. It is highly possible for IL-8 and ERβto become a new mark for the tumor molecule, which would contribute to the diagnosis and prognostic judgment of ovarian epithelial tumors, and become a new drug target.