The Expression Of Membrane Glucocorticoid Receptor On Human Peripheral Blood Mononuclear Cells And The Relationship With Different Ages

Objective To detect the expression of membrane glucocorticoid receptor on human peripheral blood mononuclear cells, and discuss the relationship between different age and mGR content, the role of membrane glucocorticoid receptor in glucocorticoid induced glaucoma.Methods The subjects were divided into three groups, in which there were 15 persons:≤15 years old group,between 3-13, the average age was 7; 15~35 years old group (including 35), between 16-35, the average age was24;>35 years old group, between 37-78, the average age was 41. Then the number of membrane glucocorticoid receptor in peripheral blood mononuclear cells, which were obtained from peripheral blood of each patient by centrifugalization, was measured by flow cytometry, and photos were taken by fluorescence microscope. The correlation was analyzed between ages and the frequency or fluorescence intensity.Results The membrane glucocorticoid receptors were expressed on the peripheral blood mononuclear cells of patients. The mGR+ cells were observed with green fluorescence with fluorescence microscope The frequencies of mGR + cells in peripheral blood mononuclear cells of three groups(≤15, 15~35, >35 years old) were 11.38±5.21%, 14.38±6.50%, 14.23±5.13%, and the fluorescence intensity of mGR + cells in peripheral blood mononuclear cells of three group was 14.71±4.39, 19.76±4.84, 16.68±4.61. The frequency or fluorescence intensity didn't correlate to the ages of patients. The mGR content was higher in the group of age between 15 and 35 years old than any other group, but only with satistical significance.with fluorescence intensity, and the group of age between 15 and 35 years old were significantly higher than the group of age≤15 years old. The age may be a factor of the susceptivity of GIG.Conclusions The membrane glucocorticoid receptors were expressed on the peripheral blood mononuclear cells of the human being. The cotent of mGR didn't correlate to the ages of patients, but the content of mGR was higher in the group of age between 15 and 35 years old than any other group.