Study Of Expression For Hypoxia-inducible Factor-1 Alpha And Iducible Nitric Oxide Synthase In Human Gliomas And The Relationship Of Their And The Formation Of Tumor Vessel

Objective To investigate the expression of hypoxia-inducible factor-1 alpha (HIF-1α)and iducible nitric oxide synthase (iNOS)in glioma,and the correlation between their and the formation of tumor vessel.Methods To collect 93 cases of human brain neurogliocytoma(49 cases of low potential malignancy group,44 cases of high potential malignancy group) and 14 cases of fresh brain tissue. HIF-1α,iNOS and VEGF expression were detected by immunohistochemistry and RT-PCR to analyze the correlation between their and the formation of tumor vessel.Results The immunohistochemistry results showed that iNOS was not expressed in the fresh brain tissue group , positive expressed in the cytoplasm of low potential malignancy group and strong expressed in the cytoplasm of high potential malignancy group.It had a statistical significancen between low potential malignancy group and high potential malignancy group(P <0.05);HIF-1αand VEGF were all expressed in the cytoplasm of normal brain tissue group and neurogliocytoma group.They were all positive weakly positive expressed in the normal brain tissue group, positive expressed in the low potential malignancy group and strong expressed in the high potential malignancy group. It had a statistical significancen withen two groups(P <0.05);Analysis of immunohistochemistry showed HIF-1α,iNOS and VEGF had a statistical significancen not only in low potential malignancy group but also in high potential malignancy grou(pP <0.05);Analysis of RT-PCR showed iNOS mRNA was not expressed in the fresh brain tissue group , positive expressed in the low potential malignancy group and strong expressed in the high potential malignancy group. .It had a statistical significancen between low potential malignancy group and high potential malignancy group(P <0.05); The RT-PCR results also showed that HIF-1αand VEGF mRNA was low expressed in the fresh brain tissue group , positive expressed in the low potential malignancy group and strong expressed in the high potential malignancy group. .It had a statistical significancen withen three groups(P <0.05)Conclusions HIF-1α,iNOS and VEGF were all participate in the tumorigenesis and development.The genetic expressions of HIF-1α,iNOS and VEGF were correlated with the malignant degree of glioma. Meanwhile, HIF-1α,iNOS and VEGF mediated the glioma cell proliferation and growth.